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Serial dilution in Widal test helps to determine the titer of antibodies in the patient's serum by observing the agglutination reaction with specific antigens. By diluting the serum sample in a stepwise manner, the titer at which agglutination occurs can be determined, providing valuable information about the presence and concentration of antibodies against Salmonella typhi and Paratyphi antigens in the blood.
What else can I help you with?
Name of the blood test for typhoid?
The blood test for typhoid is called the Widal test. It detects antibodies in the blood against the Salmonella typhi bacteria, which causes typhoid fever.
What is the purpose of serial dilution in serology?
Serial dilution in serology is used to determine the concentration of an antibody or antigen in a sample by making a series of dilutions with a known dilution factor. This allows for the creation of a standard curve to quantify the concentration of the target molecule. Serial dilution helps ensure that the concentration of the sample falls within the detectable range of the assay.
What are the advantages of serial dilution of an agar plate?
Serial dilution of an agar plate allows for the quantification of bacterial colonies by providing a range of colony counts within the plate, making it easier to count without overcrowding. It also helps to isolate individual colonies for further analysis or microbiological testing. Additionally, serial dilution can help determine the original concentration of bacteria in a sample by calculating the dilution factor.
What does typhi h and o mean in widal test?
In a Widal test, "H" refers to the flagella antigen of Salmonella typhi while "O" refers to the somatic antigen of the bacteria. The presence of antibodies against these antigens indicates a recent or current infection with Salmonella typhi, the bacterium that causes typhoid fever.
What is a ten fold serial dilution?
Serial dilution is usually 1/10 dilution. Therefore after a series of dilutions, you have a logarithmic curve of concentration (log10). Basically, if diluting 1/10 and starting off with 1 molar solution, first dilution = 0.1M, 2nd = 0.01M, 3rd = 0.001M. If making a 0.001M solution involved weighing out 0.005g of a salt for example, the error in making this solution out would be very large in comparison to weighing out 5g (1M) and diluting it 3 times by serial dilution. The benefit of it is mainly accuracy.
How do you carry out quantitative test method for Widal test?
That is a good question! The answer is simple. You do step by step dilution of the serum with probably normal (isotonic) saline solution and test the same for Widal test. This is how you do quantitative test for many other tests also.
What is false positive widal test?
What is false positive in widal test
What is the difference between widal slide test Antigen and widal tube test antigen?
The main difference between the Widal slide test antigen and the Widal tube test antigen lies in the method of reaction visualization. In the Widal slide test, the reaction is observed on a glass slide under a microscope, whereas in the Widal tube test, the reaction is seen in small test tubes. Both tests detect antibodies against specific Salmonella antigens, but differ in their setup and visual interpretation.
Will Widal Test after positive resul after treatment in Typhoid?
I mean widal test result positive after treatment
How do you read widal test?
Isolated Widal test reading does not have much clinical significance. Rising titre of Widal test done one week apart, in a febrile patient, indicates typhoid fever. You can have such rising titre of Widal test in healthy patient, after dose of typhoid vaccine.
What significant titer for widal's test?
1:80 titre is significant for determination of widal positive.
What are the diagnosis for typhoid fever?
widal test
Do antibiotics affect widal test results?
To a certain extent yes.The post antibiotics Widal Test will have Low titers of agglutinins.Hence the test may be reported as False Negative.
Name of the blood test for typhoid?
The blood test for typhoid is called the Widal test. It detects antibodies in the blood against the Salmonella typhi bacteria, which causes typhoid fever.
What is a manual method?
manual test of widal method
How you can confirm that typhoid is eradicated with treatment?
widal test.
How would you distinguish between an incubatory carrier and a chronic carrier of typhoid fever?
Widal test is negative in incubatory carrier. Widal test is always positive in chronic carrier.
