Agar is a semi solid medium used to grow bacteria.
Blood agar.
Gram Negative Bacilli
Gram-positive bacteria grows in Mannitol agar because it contains a high level of salt. This type of agar allows only certain types of bacteria to grow, making it selective.Ê
Depends on the type of bacteria. Mesophiles (the most common and commonly cultured) grow optimally at 37* Celsius (or around there, depending on the type). Thermophiles (heat loving bacteria) grow well around 60-80* C, and Psychrophiles (cold loving bacteria) grow around 15* Celsius.
Salmonella is a type of bacteria. It requires a pH of 4.4 to 9.0 (prefers 6.5 to 7.5) to grow successfully.
Blood agar is media rich in reb blood cells and the most commonly used type of media is horse blood media. It is useful for the isolation of bacteria such as Streptococci or organisms that would not readily grow on media that lack red blood cells. For the isolation of Streptococci it is also useful in observing alpha and beta haemolysis.
This type of agar is designed to grow gram-negative bacteria. It will not grow gram-positive bacteria due to a dye in the formula of the agar.
yes can mold is a type of bacteria.
Gram Negative Bacilli
Stewart's medium is a type of culture transport medium (in broth form) used to preserve bacteria until it is cultured. Stewart's medium can be found in specific culture test tubes.
Assume you are growing bacteria on a lipid medium that started at pH 7. The action of bacterial lipases should cause the pH of the medium to increase or decrease? Why?
Gram-positive bacteria grows in Mannitol agar because it contains a high level of salt. This type of agar allows only certain types of bacteria to grow, making it selective.Ê
Pathogens and spoilage bacteria can grow on deli meat left at room temperature.
4.6-7.5 Type your answer here...
In a urine culture, the lab puts a sampe of urine in conditions likely to grow the typical germs that cause urinary tract infections. If they get some to grow, they identify the bacteria in the urine.
Depends on the type of bacteria. Mesophiles (the most common and commonly cultured) grow optimally at 37* Celsius (or around there, depending on the type). Thermophiles (heat loving bacteria) grow well around 60-80* C, and Psychrophiles (cold loving bacteria) grow around 15* Celsius.
Jaimin Doshi
Its a test where a semisolid agar called Sulfide-Indole-Motility medium (or SIM medium) is inoculated with a bacteria to test for hydrogen Sulfide, Indole, and Motility of the organism. The medium is inoculated by a swab and stab type method (rub some bacteria on the surface of the medium and stab a straight hole through the medium using a straight wire with the bacteria on it). Incubate the bacteria for about 24 hours and then begin testing.... If hydrogen sulfide is present, it will react with the sodium thiosulfate in the medium and the indicator, ferric ammonium citrate, to produce ferrous sulfide which falls out of solution as a blackish precipitate. The presence of hydrogen sulfide typically means that the bacteria produces the enzyme cysteine desulfanase which breaks up the cysteine in the medium into, among other components, hydrogen sulfide. The Indole portion of the test is performed by adding Kovac's reagent to the inoculated medium. The Kovac's reagent reacts with the indole(if indole is present) to produce a pinkish-red or redish-purple ring around the top of the test tube. If indole isn't present, there will be no color change. The presence of indole means that the bacteria produces tryptophanase, an enzyme which breaks down tryptophan into smaller components, one of which being indole. The Motility aspect of the test is done by checking the medium for turbidity, or "fuzziness". If the medium has become fairly turbid throughout the medium, then the bacteria is motile. If the medium is clear and the only turbid appearance is in the stab line, then the bacteria is non-motile. Unfortunately, the motility aspect of this test typically gives false negative results. Sometimes the temperature that the bacteria was incubated at wasn't optimum for the species, sometimes the bacteria only have weak motility, sometimes the bacteria's flagella can get damaged which would impair motility, etc... The point is, this test is good if you want to know whether or not the bacteria you're testing produces tryptophanase or cysteine desulfanase. The motility aspect of the test is suspect to question, at least if the test result was negative for motility(a large amount of turbidity in the medium is a definite sign of motility and is hard to refute though).