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What will be the appearance of the paper chromatogram if a solvent of too low polarity is used to elute a polar analyte such as an artificial dye?
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Why the retention time will decrease if the polarity of mobile phase increase in normal phase HPLC?
because in normal phase HPLC mobile phase is non polar and stationary phase is polar. Most of the compound of interest are polar, if you increase the polarity of mobile phase compound of analyte will stay in mobile phase and will elute faster and retention time will be shorter.
Why internal standard you used in hplc method?
Internal standard can be used for calibration by plotting the ratio of the analyte signal to the internal standard signal as a function of the analyte concentration of the standards. This is done to correct for the loss of analyte during sample preparation or sample inlet.
The area of a blood smear that is tested is called the?
analyte
How do you prepare analyte?
The substance to be analysed normally of unknown quantity is called as analyte. it is called as sample. In titration of analytes normally we take it according to the Normality of the titrant taken to find the quantity of analyte. Sample Size = Titer Value*Normality*Molecularr(or)equivalent weight/ purity/10. This formula is expressed in terms of %.
What is Rf value?
It is the distance travelled by the sample or analyte divided by distance travelled by the solvent front in chromatography.
What is titrant and analyte?
Analyte: the substance to be analyzed by titration. Titrant: the substance (with a known concentration) added to the analyte solution to perform a titration.
Is the indicator generally added to the titrant or analyte in a titration?
Analyte is the indicator that is generally added in titration.
Is the indicator generally added to the titrant or the analyte in titration?
Analyte is the indicator that is generally added in titration.
Is the indicator generally added to the titrant or the analyte in a titration?
Analyte is the indicator that is generally added in titration.
Define the analyte in a titration?
1) The analyte is the substance in a titration whose concentration is unknown.
Why the retention time will decrease if the polarity of mobile phase increase in normal phase HPLC?
because in normal phase HPLC mobile phase is non polar and stationary phase is polar. Most of the compound of interest are polar, if you increase the polarity of mobile phase compound of analyte will stay in mobile phase and will elute faster and retention time will be shorter.
Why internal standard you used in hplc method?
Internal standard can be used for calibration by plotting the ratio of the analyte signal to the internal standard signal as a function of the analyte concentration of the standards. This is done to correct for the loss of analyte during sample preparation or sample inlet.
What is over-titration?
over titration is when too much titrant is added to the analyte in a titration procedure.
The area of a blood smear that is tested is called the?
analyte
What is the characteristics of an internal standard?
Internal Standard(IS) is similar in structure and chemical properties to the analyte of interest. We add equal amount of IS to all samples including blank and used to calculate the analyte loss while preparing the sample. IS used for calibration by plotting the ratio of analyte signal to the IS signal.
How are molecular weights found from titration?
The results of a titration will tell you the number of moles present in the analyte. If you then also know the mass of this analyte, you can calculate the molecular weight as mass/moles.
When red blood cells lyse what analyte is affected?
Potassium
