Refrigeration is not applicable to preserve sample for enzymatic assay because enzymes may lose their activity at extremely low temperatures as well. This may account for storing enzymes at 5° C or below without affecting the enzymatic activity permanently. (Anubhav, 2012)
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Refrigerating a urine sample helps to slow down the growth of bacteria that can cause the sample to deteriorate or become contaminated. This helps to maintain the integrity of the sample for accurate testing and analysis. Refrigeration also helps preserve the chemical composition of the urine for more reliable results.
When the sample size is small
There is a test kit you can buy at a refrigeration distributor that allows you to capture a refrigerant sample. With this kit you can tell acid levels and moisture content. You need to know what you're doing to get a clean, non contaminated sample.
Haemolysate is kept in ice cold water to preserve the integrity of red blood cells and prevent them from breaking down. Cooling the haemolysate slows down enzymatic reactions and helps to maintain the stability of the sample for further analysis.
The recommended proteinase K buffer recipe for optimal enzymatic activity in a biological sample typically includes Tris-HCl, calcium chloride, and sodium chloride. This buffer helps maintain the stability and activity of proteinase K, an enzyme that breaks down proteins in the sample.
A blood sample should ideally be refrigerated within 1 to 2 hours after it is drawn to preserve its integrity and prevent degradation of cellular components and analytes. Delaying refrigeration can lead to changes in the sample that may affect test results. It's important to follow specific laboratory guidelines for the type of tests being conducted, as some samples may have different requirements.
Boric acid is used as a preservative in urine sample bottles to prevent the growth of bacteria and preserve the integrity of the sample for testing. It helps to maintain the pH of the urine and keep it stable until it can be analyzed.
A fixative is a chemical solution used to preserve biological tissues or cells by stabilizing their structure and preventing decomposition. It works by cross-linking proteins and other cellular components, thereby maintaining the integrity of the sample for microscopic examination. Common fixatives include formaldehyde and ethanol, which help preserve the morphology and prevent enzymatic degradation. Fixatives are essential in histology and pathology for accurate diagnosis and research.
To preserve a sample for culture and sensitivity testing, it should be collected aseptically in a sterile container. The sample should be kept at the appropriate temperature and delivered to the lab promptly to prevent overgrowth of contaminating organisms. It is important to follow specific guidelines provided by the laboratory for proper preservation and transportation of the sample.
Yes, protein can appear in refrigerated urine if it is present in the urine sample. Refrigeration doesn't eliminate protein but can help preserve the sample for testing. If protein is detected, it may indicate a health issue, and further evaluation is typically needed. However, it's important to note that transient proteinuria can occur due to factors like stress or exercise.
Chloroform can help preserve a water sample by inhibiting the growth of bacteria and microbes that could degrade the sample. It acts as a disinfectant by disrupting the cell membranes of microorganisms, preventing them from multiplying and breaking down the organic matter in the sample. This preservation method allows for more accurate testing and analysis of the water sample over an extended period of time.