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when should the coarse wheel adjustment be used
No
You MUST use the coarse focus knob.
Yes, but only to do the most basic focusing. Keep in mind that you can break your slide if you move too close with the coarse adjustment knob and any focusing close to the slide should be done with the fine adjustment knobs. (taught HS Chemistry). Hope that helps!
You should use the lowest value lens. It is the shortest one.
A specimen should be on the top surface of the slide and place a cover-slip over it.
The coarse adjustment knob is only used with the low-power objective lens (usually 4x) because all the other ones are too long and the coarse adjustment knob may bring the slide up too qucikly, therefore it might break the slide or crush the specimen being observed. When using the medium (10x) and high (40x) power objective lens, it is best to use the fine adjustment knob to focus on the details instead.
What is the specimen?
turn the fine adjustment
You start with the lowest magnification. Once you have found the specimen and focused it, you can move it to the next higher magnification. It should still be in focus, although you might need to use the fine adjustment.
use the fine adjustment knob to get a better view of what you are looking at.For low power you can use the coarse adjustment and fine adjustmentfor high power you should only use the fine adjustment because on high power, the objective lens is too close to the slide and using the coarse adjustment may scratch the objective lens.
First of all, one should never call it high power, it is morecommonly called the "high objective", yet that is not what this question is asking. To answer the question: You use the fine adjustment knob. This knob should be located near the coarse adjustment knob, on the opposite side of the microscope (at the same height as the coarse adjustment knob), or even as a separate knob protruding from the coarse adjustment knob. The fine adjustment knob is smaller in size. You should never, ever use the coarse adjustment knob under the high objective, you could scratch the microscope slide, cover slip, high objective lens, or in a worst case scenario break the high objective lens.
when should the coarse wheel adjustment be used
Observing nature.
According to my neurons that the distance from slide to objective lens should be increase rather than decrease when coarse focusing with the scanner and LPO to obtain a clearer and more focus structure of a certain specimen.. U must ensure that the coarse adjustment knob is at it's extent and then turn on the fine adjustment knob clockwise for the easier visibility of the specimen =)) - VALERIC ACID ...sPCUTE ..=)
The coarse adjustment knob elevates the microscope's stage up and down quickly. The fine adjustment knob does the same thing but more slowly and accurately. In other words, the fine adjustment knob should require more revolutions to elevate the stage as much as the coarse adj. knob does.
It allows you to center the specimen, and if it is set up correctly, then when you switch objectives it should still be there and only need fine focusing. It also reduces the chance of crushing the specimen and/or breaking the slide or the lens as the LPO is further away from the slide than the HPO.