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use the fine adjustment knob to get a better view of what you are looking at.
For low power you can use the coarse adjustment and fine adjustment
for high power you should only use the fine adjustment because on high power, the objective lens is too close to the slide and using the coarse adjustment may scratch the objective lens.
What else can I help you with?
Why is it important to focus specimens under the Low Power Objectve before observing it under the High Power Objective?
It allows you to center the specimen, and if it is set up correctly, then when you switch objectives it should still be there and only need fine focusing. It also reduces the chance of crushing the specimen and/or breaking the slide or the lens as the LPO is further away from the slide than the HPO.
Which activity might lead to damage of a microscope and specimen?
Dropping the microscope or mishandling it can lead to damage, as well as using excessive force when adjusting the focus or moving the slides. Using improper cleaning methods or harsh chemicals can also damage the microscope and specimen.
What is the difference of what you see under LPO or scanner with what you have observed under the HPO?
LPO (Low Power Objective) provides a wider field of view and lower magnification, making it suitable for locating objects and observing their overall structure. On the other hand, the higher magnification and narrower field of view of the HPO (High Power Objective) allow for more detailed examination of specific features or structures of the specimen. The scanner objective provides the lowest magnification and widest field of view for easily locating and navigating around the specimen on the slide.
What is cover slip?
A cover slip is a thin, flat piece of glass or plastic placed over a specimen on a microscope slide. It helps protect the specimen from dust and damage, while also flattening the specimen to make it easier to view under a microscope. Cover slips are commonly used in microscopy to provide a clear, flat surface for observing specimens.
Does the switch from low power to high power change the position of the image under microscope?
No, switching from low power to high power magnifies the image but does not change its position. The position of the image remains the same as long as the slide or specimen is not moved.
What are the advantages of observing a specimen under different magnifications on a microscope?
Observing a specimen under different magnifications on a microscope allows for a more detailed examination of its structure and characteristics. This can help in identifying specific features, understanding complex structures, and gaining a deeper insight into the specimen's composition.
What objective is used when observing fresh specimen?
The objective used when observing a fresh specimen is typically a low-power objective lens, such as a 4x or 10x lens. This allows for a wider field of view and better visualization of the overall sample without the need for magnification.
What is the difference between the field of view under high and low power?
You can see less under high power because it focuses on a smaller part of the specimen, but in more detail than low power.
What is the magnification of the specimen under lpo and hpo?
The magnification of the specimen under low power optics, lpo, is 10 times and the magnification of the specimen under high power optics, hpo, depends on the power of the microscope but is usually at least 500 times or more.
What power do you always begin on when you start looking at something under a microscope?
Actually, it depends on the specimen which you are observing.. Suppose, if you are observing Tissues then you have to switch to 10X objective; but if you are observing mammalian cells or other cellular specimen then you have to switch to 45X objective...But, if you want to observe microbial cells then you have to put a drop a drop of paraffin oil on the smear made on the slide and then observe it into 100X oil immersion objectivelens...
What must be done there is too much light that illuminates the specimen you are observing under the microscope?
Turn down the light.
What must be done if there is too much light that illuminates the specimen you are observing under the microscope?
tang ina ninyo!!
Why is it important to focus specimens under the Low Power Objectve before observing it under the High Power Objective?
It allows you to center the specimen, and if it is set up correctly, then when you switch objectives it should still be there and only need fine focusing. It also reduces the chance of crushing the specimen and/or breaking the slide or the lens as the LPO is further away from the slide than the HPO.
What is the cover over the specimen called on microscope?
The cover over the specimen on a microscope is called a microscope slide cover slip. It protects the specimen from dust and damage and helps to maintain the specimen in place while observing it under the microscope.
What does it mean when a specimen is under low power magnification you can switch to high power magnification and have the specimen remain in reasonably good focus?
It means you have a good microscope.
How do you view specimen under high power on a microscope?
To view a specimen under high power on a microscope, first ensure the specimen is properly mounted on a slide and in focus under low magnification. Then, increase the magnification to high power, typically 400x or higher, using the lens adjustment knob without contacting the slide. Finally, fine-tune the focus using the fine focus knob to get a clear view of the specimen at high magnification.
Why is it necessary to use both eyes when observing under the microscope?
Using both eyes when observing under the microscope provides depth perception and enhances the clarity of the image. This allows for better spatial awareness and more accurate interpretation of the specimen being viewed.
