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When extracting DNA from a banana, the DNA must release from the cell by breaking apart or lysing the cellular and nuclear membranes. Lysing in this case is the act of breaking open the cell membranes to expose the contents. This is performed by mashing the banana and adding a detergent/salt solution.
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First step in isolating DNA from cell?
what is the first step in isolating DNA from your cells? because, in eukaryotic cell, DNA never leaves the nucleus, so the first step is to breaking the nuccleus. That answer above is not entirely correct. The first most general step in DNA extraction is cell wall and membranes not just the nucleus. Recall that a eukaryotic cell has more than one cell wall you can not obtain DNA from the nucleus without going through the first ones. Prokaryotes are essentially the same although they are much easier to extract (bacteria) since they obviously have less membrane to go through Eukaryotes are more difficult in the sense one must mechanically or chemically degrade plant cells and animal cells.
What are the benefits of extracted DNA from banana?
the benefit to extract DNA from banana IS TO PRODUCE NEW AND MORE RAPID POPULATION OF FRUITS
How the ability to isolate DNA realtes to biotechnology?
DNA isolation is the method of isolating DNA of an organism, by doing this one can study the heredity or the genes of the particular organism and can amplify the gene of interest for the beneficial applications. Thus it relates to biotechnology since the technology deals with a biomolecule!
When isolating genomic DNA how were you able to determine that the DNA was composed of large pieces and not small fragments?
Run them through a gel electrophoresis machine against a standard marker that would tell you the length of the fragments, thus large pieces from smaller pieces.
What is the result after isolating DNA?
There are several things that can be done once DNA is purified. The first thing to do is to check its purity by measuring its 260 to 280 ratio. IN this method, the absorbency of the sample is measure at 260 and 280 nm. If the ratio of these two numbers is between 1.8 and 2.0, one can consider the DNA to be pure for further applications
What is the purpose of using water bath in isolating DNA from cells?
The process of isolating DNA serves several purposes such as unlocking hereditary traits, determining paternity, and identifying unknown deceased bodies. Using water bath in isolating DNA from cells increases the yield of DNA by slowing down the enzymes that could break the DNA strand.
Are mashed potatoes fruits or vegetables?
Vegetables
Genetic engineering is really a process of?
isolating and then transferring a gene into the DNA of another organism.
Do fruits and vegetables have DNA?
Yes, all living things have DNA.
Do some fruits have more DNA than others and why?
Some fruits DO have more DNA than others. This is because of the mass and the juice of the fruit.
What are the results of the mini-prep methods by alkaline lysis?
Alkaline lysis is used for isolating plasmid DNA so one successful completion of the experiment you will see white strands of DNA.
What are the benefits of extracted DNA from banana?
the benefit to extract DNA from banana IS TO PRODUCE NEW AND MORE RAPID POPULATION OF FRUITS
First step in isolating DNA from cell?
what is the first step in isolating DNA from your cells? because, in eukaryotic cell, DNA never leaves the nucleus, so the first step is to breaking the nuccleus. That answer above is not entirely correct. The first most general step in DNA extraction is cell wall and membranes not just the nucleus. Recall that a eukaryotic cell has more than one cell wall you can not obtain DNA from the nucleus without going through the first ones. Prokaryotes are essentially the same although they are much easier to extract (bacteria) since they obviously have less membrane to go through Eukaryotes are more difficult in the sense one must mechanically or chemically degrade plant cells and animal cells.
How the ability to isolate DNA realtes to biotechnology?
DNA isolation is the method of isolating DNA of an organism, by doing this one can study the heredity or the genes of the particular organism and can amplify the gene of interest for the beneficial applications. Thus it relates to biotechnology since the technology deals with a biomolecule!
What is the extract of RNA?
RNA is the expressed form of a gene (which is DNA encoded). By isolating RNA, it is possible to determine which genes were being expressed and to what relative (or even absolute) level.
When isolating genomic DNA how were you able to determine that the DNA was composed of large pieces and not small fragments?
Run them through a gel electrophoresis machine against a standard marker that would tell you the length of the fragments, thus large pieces from smaller pieces.
Role of RBC lysis solution in DNA isolation?
When isolating DNA from blood, white blood cells (WBC's) are the target. This is because RBC's do not contain a nucleus and therefore do not contain DNA. The function of the lysis buffer is to help in the lysis (or breaking) of white blood cells. WBC's must first be lysed so that the DNA may be released from inside the cell.
