An analytical method for the profiling and quantitative determination of carotenoids in bacteria is described. Exhaustive extraction of the pigments from four selected bacterial strains required treatment of the cells with potassium hydroxide or liquefied phenol or both before the addition of the extracting solvent (methanol or diethyl ether). The carotenoids in the extracts were separated by nonaqueous reversed-phase liquid chromatography in conjunction with photodiode array absorption detection. The identity of a peak was considered definitive only when both its retention time and absorption spectrum, before and after chemical reactions, matched those of a reference component. In the absence of the latter, most peaks could be tentatively identified. Two examples illustrate how in the analysis of pigmented bacteria errors may result from using nonchromatographic procedures or liquid chromatographic methods lacking sufficient criteria for peak identification. Carotenoids of interest were determined quantitatively when the authentic reference substance was available or, alternatively, were determined semiquantitatively http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=203224
there are different types like gas chrom. and thinlayer chrom Answer: There are two types of chromatography:liquid chromatography gas chromatography
What is the precedure for calibration of gas chromatography? How calibration is perform for gas chromatography? What is the precedure for calibration of gas chromatography? How calibration is perform for gas chromatography?
There is no such thing as grass chromatography. there is only High Performance Liquid Chromatography, Gas Chomatography, and Paper Chromatography.
Chromatography is the method used to separate a mixture of dyes or inks.
it can be used in everyday life by liquid chromatography, gas chromatography, thin-layer chromatographyand paper chromatography.
Paper chromatography can be used to separate pigments in plants. The pigments are separated into chlorophylls (greenish colors) and carotenoids (yellowish colors).
The carotenoids appear to have many health benefits, but more research is required to confirm many of the health effects seen so far and to identify additional benefits.
C40H56
carotenoids :]
Place leaf in beaker and heat with Bunsen burner until water has discolored and boiled, then place leaf in test tube filled methylated spirits and place in same beaker as before with the burner turned OFF. Methylated spirits boils at around 80 degrees Celsius so when placed in heated water it will boil. This process will separate the chlorophyll (pigment) in the leaf.
there are different types like gas chrom. and thinlayer chrom Answer: There are two types of chromatography:liquid chromatography gas chromatography
These are carotenoids
According to scientists, carotenoids contain only carbon and hydrocarbon atoms and are usually found in carrots and apricots. Also, carotenoids is an excellent source of vitamin A.
1. thin -layer chromatography 2. gas chromatography 3. liquid chromatography
What is the precedure for calibration of gas chromatography? How calibration is perform for gas chromatography? What is the precedure for calibration of gas chromatography? How calibration is perform for gas chromatography?
There is no such thing as grass chromatography. there is only High Performance Liquid Chromatography, Gas Chomatography, and Paper Chromatography.
John Thomas Landrum has written: 'Carotenoids' -- subject(s): Carotenoids