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Where could chromatography be used?

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Anonymous

16y ago
Updated: 8/17/2019

Chromatography comprises a wide variety of analytical methods and is a common tool for chemists. Chromatography can be used in two main ways: preparatory work and analysis. In preparatory work, the chromatography column can be used to separate one type of molecule from another based on the characteristics of that molecule. An example would be taking the cellular components of digested cells and separating the DNA out from all of the other components. In analysis, an unknown sample can be sent through a chromatography column which is set up to an analyzer (for which there are many kinds). Molecules separate based on their characteristics and the detector can record how long it takes them to leave the column. Standards (solutions that have a known chemical in them) can be used for comparison. If a standard and an unknown compound both move through the same column in the same amount of time, it is reasonable to assume that this is what the unknown is. Chromatography is used in all forms of science and new methods and techniques have been created to enhance the detection limits and usefulness of chromatography. The details of chromatography are quite in depth as this is a complex kind of scientific analysis.

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16y ago

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How could you put chromatography in a sentence?

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An experiment was performed in lab that was supposed to produce aspirin. How could chromatography be used to tell if aspirin was actually the product of the reaction?

Chromatography could be used to separate the components of the reaction mixture and identify if aspirin is present by comparing the retention time of the product to that of a known aspirin standard. If the retention time matches, it indicates the presence of aspirin in the reaction mixture. Additionally, chromatography can help determine the purity of the aspirin product by analyzing the intensity of the peak corresponding to aspirin.