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DCPIP oxidized is higher in energy
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∙ 11y ago
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How does dcpip in the hill reaction affect absorbance?
DCPIP is a blue colour when its oxidized and when it is reduced it turns into a colourless solution. DCPIP replaces NADPH the final electron acceptor in the light dependent reaction. So basically, as the reaction continues, the more DCPIP is reduced and the absorbance decreases.
Explain why DPIP changes from blue to colorless?
It happens due to redox reactions. Oxidized DCPIP is blue, while reduced DCPIP is colorless. More specifically theres a Nitrogen atom joining two benzyl groups, that, when reduced, changes a double bond to a single bond, forcing several carbon bonds in the entire left benzyl ring to change conformation. This makes the molecule reflect light differently and accounts for the change in color perceived.
What is DCPIP?
The fullform of DCPIP is 2,6- dichlorophenolindophenol.
Are boiled chloroplasts able to reduce DPIP?
Boiling chloroplasts prevents the DPIP from being reduced because the enzymes for photosynthesis are no longer present in the chloroplasts. DPIP is reduced from blue to colorless when light strikes the chloroplasts and the electrons are boosted to a higher energy level. Since photosynthesis cannot be performed by the denatured chloroplasts, the DPIP cannot be reduced.
What are the risks of DCPIP?
hmmm.. Safety gloves should be worn in case of a spillage of DCPIP but it's optional. Spilling DCPIP may cause staining on the skin or the surface you are working at. Risks of DCPIP Are extremely low. Do not consume the DCPIP or purposely inhale the substance in case of any dangers or problems related with doing so.
Is dcpip harmful?
no its not
How do you prepare DCPIP solution?
1 % DCPIP solution can be prepared by dissolving 1g of the dye in 100cm3 of water
Are chloroplast necessary for reduction of DCPIP and why?
Chloroplasts release electrons when stimulated by light the DCPIP accepts the electrons and becomes colourless
What is the mass of DCPIP powder needed to form 300 mL of 1percent DCPIP solution and all measurements in grams and millilitres?
3 g
What is a DCPIP indicator for?
It can be used to test for vitamin C
What colour will the DCPIP test give if it is positive?
it will become colourless.
How does DCPIP work as an indicator in the detection of Vitamin C?
Yes it does (I know because i did the test on orange juice) The more Vitamin C something has in it the less drops of that thing (in my case the orange juice) is needed for the DCPIP to go pale yellow. It is an awesome test! I would try it ( only if you have DCPIP and lots of different types of orange juice! Of course!)
