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It happens due to redox reactions. Oxidized DCPIP is blue, while reduced DCPIP is colorless.

More specifically theres a Nitrogen atom joining two benzyl groups, that, when reduced, changes a double bond to a single bond, forcing several carbon bonds in the entire left benzyl ring to change conformation. This makes the molecule reflect light differently and accounts for the change in color perceived.

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Q: Explain why DPIP changes from blue to colorless?
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In lab 4, DPIP is an electron acceptor that takes the place of NADP+. When DPIP is reduced, it changes from blue to colorless so you can see that the Calvin cycle is running.


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If you are referring to the "Plant Pigments and Photosynthesis" Lab Then, the DPIP is used to substitute NADP+. In photosynthesis, electrons are normally transferred to NADP+. However, DPIP will take its place in this experiment. DPIP is normally blue. When it is reduced, or gains electrons, it will turn colorless. DPIP is used to show that photosynthesis is taking place.


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Boiling chloroplasts prevents the DPIP from being reduced because the enzymes for photosynthesis are no longer present in the chloroplasts. DPIP is reduced from blue to colorless when light strikes the chloroplasts and the electrons are boosted to a higher energy level. Since photosynthesis cannot be performed by the denatured chloroplasts, the DPIP cannot be reduced.What_is_the_effect_of_boiling_chloroplasts_on_the_subsequent_reduction_of_DPIP


What is the function of DPIP in this experiment?

Is this an AP lab?The DPIP replaces NADP+.In photosynthesis, NADP is reduced to NADPH, but NADP is colorless.DPIP replaces the NADP. When DPIP is reduced, it changes from blue to clear. DPIP is used to show photosynthesis taking place.


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Boiling chloroplasts prevents the DPIP from being reduced because the enzymes for photosynthesis are no longer present in the chloroplasts. DPIP is reduced from blue to colorless when light strikes the chloroplasts and the electrons are boosted to a higher energy level. Since photosynthesis cannot be performed by the denatured chloroplasts, the DPIP cannot be reduced.


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