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Boiling chloroplasts prevents the DPIP from being reduced because the enzymes for photosynthesis are no longer present in the chloroplasts. DPIP is reduced from blue to colorless when light strikes the chloroplasts and the electrons are boosted to a higher energy level. Since photosynthesis cannot be performed by the denatured chloroplasts, the DPIP cannot be reduced.

What_is_the_effect_of_boiling_chloroplasts_on_the_subsequent_reduction_of_DPIP

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Q: What is the effect of boiling the chloroplast on the subsequent?
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