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the distance between the specimen and the objective lens
A microscope should not be focused by moving the objectives and the slide closer together because it will affect the working distance. It is the optimal distance between objective lens and the upper surface of the slide.
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the distance between the lenses
A confocal microscope works by a laser shining on two mirrors mounted on motors which allows it to scan the sample then the emitted light is focued through the pinhole to a detector which relays an image to the lens.
the distance between the specimen and the objective lens
There's a knob on The side that allows you to adjust the proper height of the lense
Decrease.
The monocular has 3 objective lenses but the stereo microscope has only 2 objective lenses
It's called the "working distance" and is defined as, "the distance from the front lens element of the objective to the closest surface of the coverslip when the specimen is in sharp focus."
A microscope should not be focused by moving the objectives and the slide closer together because it will affect the working distance. It is the optimal distance between objective lens and the upper surface of the slide.
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As the magnification of the objective increases, the FOV decreases
maintain distance between your eye and and eye piece of the microscope it helps
the distance between the lenses
Working distance or WD is the distance between each objective and the specimen, when precise focus of the specimen is obtained.