Erwin Chargaff noticed that adenine and thymine occur in a 1:1 ratio and cytosine and guanine occur in a 1:1 ratio. James Watson and Francis Crick used this discovery, among others, to develop their model of the DNA molecule.
Erwin Chargaff, an Austrian biochemist, first developed rules about base composition in DNA in the early 1950s. Chargaff's rules stated that the amount of adenine is roughly equal to thymine, and the amount of guanine is roughly equal to cytosine in DNA molecules.
recombinant DNA
recombinant DNA
The scientist who first developed rules regarding base composition in DNA was Erwin Chargaff. Chargaff's rules state that in a double-stranded DNA molecule, the amount of adenine (A) equals that of thymine (T), and the amount of cytosine (C) equals that of guanine (G). This foundational work laid the groundwork for understanding the base pairing in DNA and contributed to the discovery of the double helix structure by Watson and Crick.
DNA ligase is the enzyme that is responsible for joining Okazaki fragments on the lagging strand during DNA replication. It catalyzes the formation of phosphodiester bonds between the fragments, sealing the gaps in the newly synthesized DNA.
The last step in the production of a recombinant DNA plasmid is joining the DNA. This is done by adding DNA ligase to joint DNA fragments.
James Watson and Francis Crick developed the double helix model of DNA in 1953.
DNA ligase is an enzyme that plays a crucial role in the process of DNA replication and repair by joining together the ends of DNA strands to form a continuous strand.
The newly created DNA strand binds to the parent DNA strand with the help of ligase enzyme.
In DNA replication, DNA polymerase III is the enzyme which joins the DNA nucleotides together via phospodiester bonds.DNA Ligase is the enzyme that seals gaps in DNA during DNA Replication.DNA Ligase is the enzyme that seals gaps in DNA during DNA Replication.
The rules have developed significantly since 1837. The rules are now openly avaiable on line at the IRB site and include reviewed rules and those that are being developed through actual trial play.
to provide rules of engagement