Many flew more missions than that.
Kamikaze pilots
In World War II the Axis powers turned their airplanes into weapons during Kamikaze missions. The Japanese are the most famous for this. Kamikaze missions were suicide missions, so the pilots flew to a target and aimed their plane to the ground and BOOM. Kamikaze missions were also prevalent in sea battles where one plane could take out an entire ship.
US Airmen flew the missions to drop supplies over the Berlin Wall.
WWII
5 operational Space Shuttles were built, Columbia, Challenger, Discovery, Atlantis and Endeavour. Challenger was destroyed in an explosion during launch and Discovery was destroyed when it disintegrated during re-entry. Neither accident was caused by the orbiter itself. They all flew mission of national or scientific interest. Columbia flew 28 missions. Challenger flew 10 missions. Discovery flew 39 missions. Atlantis flew 33 missions. Endeavour flew 25 missions.
It was the nickname of a B17 he flew in WWII
Many flew more missions than that.
17
Space Shuttle Columbia flew 28 missions.
7 astronaut did
Kamikaze pilots
As a warplane, capable of doing long-range bombing missions. We needed such an aircraft, the the B17 and B24 are both a reflection of that need.
I would have to say it was the United States Army Air Force and the United States Naval air force that flew the most missions. Here is my reasoning. The US was not only flying missions in Europe they were also flying missions in the Pacific theater. So they would add all up all those missions and I can be fairly certain they out numbered the Royal Army Air Force mission total. Now which particular group flew the most? I heard it was the 9th Air Force and one of the RAF groups but some of the 9th Air Force was sent to the Pacific so I still think they would win the title of flying the most missions.
If they were on official missions yes.
Harrison "Jack" Schmitt PhD flew on one mission, the Apollo 17 lunar landing.
C130