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yes
Yes, an antagonist can be a disease if it acts against the protagonist (person or organism) by causing harm or hindrance to their goals or well-being. Diseases can create conflict and obstacles in a storyline, making them a viable antagonist.
The antagonist is whatever is in conflict with the main character and preventing them from obtaining their goals. All fiction books should have an antagonist of some sort, yes.
yes
Yes.
yes
the person who frustrates or stands in the way of the main character or the protagonist.an antagonist is someone or something that opposes the protagonist of a story
We don't know the operation.
Yes she is the villain and antagonist of Pirates!
Yes he is
yes
Yes because he was fighting against the pirates.