Jane Lynch
She's straight in real life, but she played the role of a lesbian aunt of Kurt on Glee. It was only acting, though.
No, Dianna Agron, who plays Quinn on Glee, is not disabled in real life.
The character "Rachel" from Glee is portrayed by Lea Michele in real life.
No she is not.
Santana and Britney
No
"Duets"
NO
Nope.
In real life? Or in the TV series
no in real life i think she is single, but in glee she is dating artie
Yes, in real life Chris Colfer is Gay.