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The villain for Murder Under the Microscope 2009 was Habitat Clearing.

Backpackers were not told what to pull out for the town festival, so all of the Blue Banded Bee's nests were destroyed around the Caboolture River.

The list of possible villains was :

- Acid surface soils - Bird flu virus - Cane toad - Chemical spill - Rubbish Dumping - Genetically modified crops - Lantana - Habitat clearing - Recreational Activities - Rising Temperatures - Impervious surfaces - Pesticides - Soil depletion - Varroa mite - Sewage

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