Interesting and very difficult to put into x amount of letters.
Restriction Enzymes
Gene cloning is the technique of recombinant DNA technology in which a desired gene of interest having a striking characteristic feature is cloned. The gene may be selected because it appears to influence the organism in a striking manner, or to determine the role of the gene in the organism. Genes can be clones for industrial purposes, for instance the production of vaccines and insulin, or for research purposes, to determine what the role of the gene is. Gene cloning requires a basic knowledge of the gene's sequence, or flanking sequences. Genes can be cloned using polymerase chain reaction (PCR), if the sequence is known, or by cutting genomic DNA with restriction enzymes (to create smaller chunks of DNA). Usually, once a fragment containing gene has been identified using restriction enzymes, it is sequenced and PCR is used to isolate the specific sequence within the fragment.
Restriction enzymes are the bacteria's form of an 'immune system' against viruses (which can infect bacteria). When viruses try to insert their own DNA into a bacteria's genome, the restriction enzymes detect this foreign DNA and cut it out so that the viruses can't replicate and kill the cell.
bacteria
Enzymes
restriction enzymes
Restriction Enzymes
Scientists have taken restriction enzymes out of bacteria; restriction enzymes are used to cut DNA at cut sites. Also, they insert genes into bacteria to study them.
restriction endonuclease
Gene cloning is the technique of recombinant DNA technology in which a desired gene of interest having a striking characteristic feature is cloned. The gene may be selected because it appears to influence the organism in a striking manner, or to determine the role of the gene in the organism. Genes can be clones for industrial purposes, for instance the production of vaccines and insulin, or for research purposes, to determine what the role of the gene is. Gene cloning requires a basic knowledge of the gene's sequence, or flanking sequences. Genes can be cloned using polymerase chain reaction (PCR), if the sequence is known, or by cutting genomic DNA with restriction enzymes (to create smaller chunks of DNA). Usually, once a fragment containing gene has been identified using restriction enzymes, it is sequenced and PCR is used to isolate the specific sequence within the fragment.
The difference between cloning genes and cloning a mammal is that when a gene is cloned, it is typically removed from a DNA sequence and inserted into an organism. The cloning of a mammal, however, is when a somatic cells from the mammal are cloned to produce a "copy" of the mammal.
Restriction enzymes are the bacteria's form of an 'immune system' against viruses (which can infect bacteria). When viruses try to insert their own DNA into a bacteria's genome, the restriction enzymes detect this foreign DNA and cut it out so that the viruses can't replicate and kill the cell.
The enzymes being used to cut the vector open allowing the insertion of the gene of interest. Once the gene has integrated into the host plasmid it can grown in a media conducive to which ever particular strain of bacteria it is. As the transformed bacteria grow in colonies each cell will possesses the gene that was inserted. Now there is a large quantity of "cloned" genes which can be isolated back out and examined. - Rough summary-
Cloning
Cloning
Cloning
bacteria