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One of the strongest reasons he had for thinking natural selection was the driving force of evolution in fact he started his book with this concept, was artificial selection. Breeders had long know that traits were heritable and they could select for the traits they wanted by just mating the favored organisms. From this example Darwin knew that acquired traits were neither heritable or selectable.

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Q: Why did Darwin believe organisims evolved using natural selection instead of required traits?
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