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There were ethical and practical reasons why Harlow could not use humans in experiments to identify the nature of attachment between human infants and their mothers.

Animals were not protected by the same guidelines on ethics. Therefore, Harlow could use monkeys in his experiments. The macaques are close to humans in evolutionary terms and in many behavioural traits so Harlow could gain insight which could transfer to human models.

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Q: Why did Harlow use monkeys instead of humans?
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