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The reason we use IS6110 for the identification of TB using procedures such as RFLP and Spoligotyping for several years now is because this is a genetic insertion sequence (IS) or element that is found exclusively within the members of the Mycobacterium tuberculosis complex (MTBC). Some researchers have found IS6110-like elements in some other mycobacteria such as M. smegmatis but because smegmatis do not cause TB, its clinical importance is lesser. This info was taken from "IS6110 is found in non-M. tuberculosis complex species" by Coros A, DeConno E, and Derbyshire KM, from the Wadsworth Center, NY, NY, (AMS March 7, 2008)

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Q: Why do we target IS 6110 for the identification of Mycobacterium tuberculosis?
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