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A stereo microscope shows two slides side by side at the same time and is used for comparison. A compound microscope only shows one slide.
1. Ocular Lens (Eyepiece) - where you look through to see the image of your specimen. Magnifies the specimen 10X actual size. 2. Body tube - the long tube that supports the eyepiece and connects it to the objectives. 3. Nosepiece - the rotating part of the microscope at the bottom of the body tube; it holds the objectives. 4. Objective Lenses - (low, medium, high). Depending on the microscope, you may have 2, 3 or more objectives attached to the nosepiece; they vary in length (the shortest is the lowest power or magnification; the longest is the highest power or magnification). 5. Arm - part of the microscope that you carry the microscope with; connects the head and base of the microscope. 6. Coarse Adjustment Knob - large, round knob on the side of the microscope used for "rough" focusing of the specimen; it may move either the stage or the upper part of the microscope. Location may vary depending on microscope - it may be on the bottom of the arm or on the top. 7. Fine Adjustment Knob - small, round knob on the side of the microscope used to fine-tune the focus of your specimen after using the coarse adjustment knob. As with the Coarse Adjustment Knob, location may vary depending on the microscope. 8. Stage - large, flat area under the objectives; it has a hole in it (see aperture) that allows light through; the specimen/slide is placed on the stage for viewing. 9. Stage Clips - clips on top of the stage which hold the slide in place. 10. Aperture - the hole in the stage that concentrates light through the specimen for better viewing. 11. Diaphragm - controls the amount of light going through the aperture; may be adjusted. 12. Light or Mirror - source of light usually found near the base of the microscope; used to direct light upward through the microscope. The light source makes the specimen easier to see.
The knobs on the side of a microscope are the focus knobs. The large one is the course focus, and the smaller one is the fine focus.
The fine adjustment on a microscope is the smaller of the two knobs on the side. It is used after the coarse adjustment, which moves the platform up/down. It is mainly used to focus.
For a light transmission microscope, a thin sample is needed in order for the light to pass through the sample from the light source on the other side. However, you can use a dissection microscope (another type of light microscope) which illuminates the sample from above, eliminating the need for a thin sample size. As the name suggests, this is normally used in dissections, and is of low magnification.
Sometimes, if the stage is set high enough, the objective lens can hit or scratch the slide when it's changed, because each objective lens is a different length. From the back of the microscope, you cannot see if the lens is going to hit the slide or not, and run the risk of disturbing your sample. This problem can be prevented by looking from the side and stopping if it looks like the lens is going to hit the slide.
Sometimes, if the stage is set high enough, the objective lens can hit or scratch the slide when it's changed, because each objective lens is a different length. From the back of the microscope, you cannot see if the lens is going to hit the slide or not, and run the risk of disturbing your sample. This problem can be prevented by looking from the side and stopping if it looks like the lens is going to hit the slide.
Sometimes, if the stage is set high enough, the objective lens can hit or scratch the slide when it's changed, because each objective lens is a different length. From the back of the microscope, you cannot see if the lens is going to hit the slide or not, and run the risk of disturbing your sample. This problem can be prevented by looking from the side and stopping if it looks like the lens is going to hit the slide.
The different levels of magnification. It makes it easier for you to recognize the magnification you want without having to read the numbers on the side.
A stereo microscope shows two slides side by side at the same time and is used for comparison. A compound microscope only shows one slide.
Nosepiece
A stereo microscope shows two slides side by side at the same time and is used for comparison. A compound microscope only shows one slide.
The stereoscopic microscope provides a right side up image
1. Ocular Lens (Eyepiece) - where you look through to see the image of your specimen. Magnifies the specimen 10X actual size. 2. Body tube - the long tube that supports the eyepiece and connects it to the objectives. 3. Nosepiece - the rotating part of the microscope at the bottom of the body tube; it holds the objectives. 4. Objective Lenses - (low, medium, high). Depending on the microscope, you may have 2, 3 or more objectives attached to the nosepiece; they vary in length (the shortest is the lowest power or magnification; the longest is the highest power or magnification). 5. Arm - part of the microscope that you carry the microscope with; connects the head and base of the microscope. 6. Coarse Adjustment Knob - large, round knob on the side of the microscope used for "rough" focusing of the specimen; it may move either the stage or the upper part of the microscope. Location may vary depending on microscope - it may be on the bottom of the arm or on the top. 7. Fine Adjustment Knob - small, round knob on the side of the microscope used to fine-tune the focus of your specimen after using the coarse adjustment knob. As with the Coarse Adjustment Knob, location may vary depending on the microscope. 8. Stage - large, flat area under the objectives; it has a hole in it (see aperture) that allows light through; the specimen/slide is placed on the stage for viewing. 9. Stage Clips - clips on top of the stage which hold the slide in place. 10. Aperture - the hole in the stage that concentrates light through the specimen for better viewing. 11. Diaphragm - controls the amount of light going through the aperture; may be adjusted. 12. Light or Mirror - source of light usually found near the base of the microscope; used to direct light upward through the microscope. The light source makes the specimen easier to see.
The knobs on the side of a microscope are the focus knobs. The large one is the course focus, and the smaller one is the fine focus.
phase contrast
Sometimes, if the stage is set high enough, the objective lens can hit or scratch the slide when it's changed, because each objective lens is a different length. From the back of the microscope, you cannot see if the lens is going to hit the slide or not, and run the risk of disturbing your sample. This problem can be prevented by looking from the side and stopping if it looks like the lens is going to hit the slide.