flouride inhibits an enzyme involved in glycolysis. In order to prevent glycolysis in the drawn blood, tubes containing flouride are used.
the purpose of distilled water is so that chemicals in the water do not interfere with the experiment :)
I'm 90% sure that a FBS(fasting glucose) is drawn into a SST(serum seperator tube) A.k.a. "tiger top".
glucose does
because if we do not have glucose we cannot produce a glucose..
Glucose. A reducing sugar.
Sodium fluoride can be used as a preservative for blood samples (for the glucose determination) avoiding glycolysis.
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Chemistry
decreases blood glucose, as fluoride inhibits enolase enzyme in glycolysis, and there is an accumulation of phosphenolpyruvate and no pyruvate being produced, thus no energy.
It is commonly used for glucose and lactate tests. The fluoride is used to stop/slow down processes in the red bloodcells(also in the other cells, but the erythrocytes have the biggest influence in this matter). Processes like the citric acid cycle, which uses glucose to produce energy in the form of ATP. In this process lactate is formed along with ATP. In other words, the glucose concentration in the plasma will decrease, and the lactate concentration will increase. When the blood is being obtained it will take about 30 minutes for the fluoride to start working.
From the BD web siteWhat is the glycolytic inhibitor in the gray top tube?Sodium fluoride acts as the glycolytic inhibitor and prevents the cells in the blood from utilizing the glucose.
Glucose Tolerance Test....not sure what tube you would collect it with....maybe a yellow top....
Simply put, the enzymatic method ( eg. glucose oxidase and hexokinase etc.) is the best method for blood glucose concentration because of its specificity. This yeilds maximum specificity for glucose estimation even in a comlpex mixture. Sherry-Ann Ramnarine-Phillips
In the presence of heat and acetic acid, ortho-toluidine (also called o-toluidine) reacts rapidly with aldohexoses (mostly glucose) to form a green-colored complex that can be measured at 630 nm.
Glucose Tolerance Test Grey Top Tube.
It could be either red top (to clot it) or Grey top (sodium fluoride or potassium oxalate added) which prevents RBC's from metabolising the glucose in the blood. There is also another one to separate the serum but I forgot the color. It might be "tiger top" or speckled.
I suppose that barium chloride cause the greatest drop of the freezing point.