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If they were not dried well then any water on the specimen could refract or reflect the light coming at it...and so distorted images would be the result.
An EM is basically of 2 types. Scanning Electron Microscope and Transmission Electron Microscope. Yes definitely it is used to magnify and resolve specimens but a living specimen cannot be used. Only dead are used. This is due to certain disadvantages that come along with this microscope. If need further details over the microscope uses and types then let me know.yea...i completely agree wif yuh... :)
Because an air bubble, trapped between the slide and cover plate, shows as a dark'blob' under magnification. Using a drop of water to 'seal' the slide & cover plate together excludes the air - making the specimen more visible to the user.
For a light transmission microscope, a thin sample is needed in order for the light to pass through the sample from the light source on the other side. However, you can use a dissection microscope (another type of light microscope) which illuminates the sample from above, eliminating the need for a thin sample size. As the name suggests, this is normally used in dissections, and is of low magnification.
Specimens are placed in a vacuum (high vacuum, requiring a cryopump to get to 10^-7 torr. Then the specimen is bombarded with an electron beam (like getting a shock). Not likely that something could survive that.
If they were not dried well then any water on the specimen could refract or reflect the light coming at it...and so distorted images would be the result.
When you need to observe a live specimen
well, if you are trying to see something microscopic, you can look though a microscope to see it. but, if the microscope is in a very high scope, ex 1000x, you need to adjust the height of the scope using a knob on the side.
i need the same answer...-.-
A phase-contrast microscope (or phase-difference microscope or phase microscope) allows better viewing of clear specimens that usually need to be stained before you can make out the various parts. Staining is a time consuming process and kills the cells being viewed. With a phase-contrast microscope, living cells can be studied as they go through the cell division cycle.HOW IT WORKSAs light goes through a transparent specimen, there may not be anything interesting to see. However, the light undergoes phase changes as it passes through the different parts of the specimen. Unfortunately, the human eye does not notice phase changes. A phase-contrast microscope converts these unnoticed phase changes into differences in brightness, which the human eye sees very well. The darker and lighter parts of the specimen make it very visible.This also works on reflected light microscopes.
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An EM is basically of 2 types. Scanning Electron Microscope and Transmission Electron Microscope. Yes definitely it is used to magnify and resolve specimens but a living specimen cannot be used. Only dead are used. This is due to certain disadvantages that come along with this microscope. If need further details over the microscope uses and types then let me know.yea...i completely agree wif yuh... :)
It depends on what the specimen is. If for example - the specimen is a person's finger-print, then low magnification is sufficient. However - if the specimen is a sample of blood, a higher magnification would be needed to show individual blood cells.
Because the smallest wavelength of visible light we can see is around 400 nm. Something 200 nm would need an electron microscope to be seen.
A phase-contrast microscope (or phase-difference microscope or phase microscope) allows better viewing of clear specimens that usually need to be stained before you can make out the various parts. Staining is a time consuming process and kills the cells being viewed. With a phase-contrast microscope, living cells can be studied as they go through the cell division cycle.HOW IT WORKSAs light goes through a transparent specimen, there may not be anything interesting to see. However, the light undergoes phase changes as it passes through the different parts of the specimen. Unfortunately, the human eye does not notice phase changes. A phase-contrast microscope converts these unnoticed phase changes into differences in brightness, which the human eye sees very well. The darker and lighter parts of the specimen make it very visible.This also works on reflected light microscopes.