Well it depends on the solvent you are using, but lets assume you use pentane as the solvent for fluorene and diethyl ether as the solvent for fluorenone. Used in this order, fluorene should actaully move slower down the column due to the the polarity of the alumina and the nonpolarity of the solvent (pentane). Since fluorene is less polar so, remember the rule like dissolves like? Well, this kind of applies to chromatography wherein rather than dissolve just replace it with moves with, so like moves with like. That being said the pentane will "carry" the fluorene through the alumina slower than the latter (which I will explain). Fluorenone is polar because of its C=O bond, that being said the dielectric constant of diethyl ether is 4.3 which means it has intermediate polarity (remember that pentane has a dielectric constant of 2.1 I think, so it is nonpolar). Since the alumina, diethyl ether, and the fluorenone are all polar, the fluorenone will travel faster through the alumina than would the fluorene, because there is no attraction between all these polar compounds which will allow it to move faster, rather than a nonpolar and polar chemical having an attration towards each other and thus moving more slowly.
Hope this helps,
Branden
fluorenone is more polar compound than fluorene, at the same time silica gel is polar compound, then polar compound will that is fluorenone will be retained or absorbed in the stationary phase whereas fluorene will be eluted out
be cause of its polarity
Column chromatography, is a broad term for all column chromatography methods, but is also synonomous with Gravity fed methods. Flash chromotography refers specifically to a column in which the eluant (or mobile phase) is moved through the column under pressure (using a hand pump for small scale, or a pressurised gas for a larger scale), the name Flash is derived from how much faster it is to run a column under pressure than via gravity.
Flash chromatography uses pressure (under 10 psi) to pump solvent down a column at a rate faster than gravity would provide. Vacuum chromatography uses a vacuum at the bottom of the column to pull solvent through. Both can be performed with standard glass columns, but usually vacuum chromatography is done with a silica filled vacuum funnel instead as a rough purification technique.
•Descending chromatography is faster because gravity helps the solvent flow.
The property of solvent determines the rate of migration of solute i.e., if the solvent is nonpolar, nonpolar molecules will move faster and if the solvent is polar, than polar molecules will move faster during separation.
Dry Column Chromatography (DCC) is a fast, easy, and efficient method for separating and/or purifying industrial quantities of compounds.
One is faster and more flexible, the other is a bit heavier
Column chromatography, is a broad term for all column chromatography methods, but is also synonomous with Gravity fed methods. Flash chromotography refers specifically to a column in which the eluant (or mobile phase) is moved through the column under pressure (using a hand pump for small scale, or a pressurised gas for a larger scale), the name Flash is derived from how much faster it is to run a column under pressure than via gravity.
Flash chromatography uses pressure (under 10 psi) to pump solvent down a column at a rate faster than gravity would provide. Vacuum chromatography uses a vacuum at the bottom of the column to pull solvent through. Both can be performed with standard glass columns, but usually vacuum chromatography is done with a silica filled vacuum funnel instead as a rough purification technique.
•Descending chromatography is faster because gravity helps the solvent flow.
TLC. The mobile phase is a liquid, the stationary phase is a solid. Useful for seperating and comparing mobility of solids and some liquids dissolved in the mobile phase by their affinities to the solid phase relative to the mobile phase. GLC. The mobile phase ia s gas, the stationary phase is a liquid on a solid support. same concept as TLC. useful for seperating gases by their affinities to the stationary phase...the mobility can then be compared to known compounds for possible identification.
Smooth marble column
The property of solvent determines the rate of migration of solute i.e., if the solvent is nonpolar, nonpolar molecules will move faster and if the solvent is polar, than polar molecules will move faster during separation.
Dry Column Chromatography (DCC) is a fast, easy, and efficient method for separating and/or purifying industrial quantities of compounds.
Chromatography is a technique that separates molecules from each other on the basis of their solubility in particular solvents. As a nonpolar solvent moves up the chromatography paper, the pigment moves along iwth it. The more non-polar a pigment, the more soluble it is in a nonpolar solvent, and the faster and father it proceeds up the chromatography. Pg 94, laboratory 8.1, Inquiry into Life, Sylvia S. Mader, laboratory manual, 12th edition
When a mixture of mobile phase and sample to be separated are introduced from top of the column, the individual components of mixture move with different rates. Those with lower affinity and adsorption to stationary phase move faster and eluted out first while those with greater adsorption affinity move or travel slower and get eluted out last. The solute molecules adsorb to the column in a reversible manner. The rate of the movement of the components is given as follows R= Rate of movement of a component / Rate of movement of mobile phase. i.e. it is the ratio of distance moved by solute to the distance moved by solvent.
gas chromatographt (GC) and High Performance Liquid Chromatography (HPLC) are different , and to understand why you must think about what chromatography is: Chromatography in its simplest form is like putting ink on blotting paper and watching the colours separate. Liquid chromatoraphy uses a "column" which is made from bare or bonded silica, it separates a mixture of compounds by how polar they are. You can use a gradient of different solvents. GC also uses a column, but it is a capillary column and instead of using a liquid to carry your mixture which needs to be separated it uses a carrier gas, like nitrogen. You can vary the temperatures in both LC and GC to aid better resolution. GC is used for more volatile compounds and LC is used more less volatile. HPLC usually refers to reversed phase, normal phase is where the column is vare silica which is very polar. Bonded silica is bonded with hydrocarbons which is non polar. The thing to remember is that "like attracts like" so if the column in non polar, the compound to elute first will be the most polar. To summarise, they are both separation techniques, one uses gas and the other liquid. You would choose which one to uese depending on how volatile the compounds which you want to separate are. Vishal Bobade NCL,Pune
It is simply that you need to be in equilibrum in pressure in the column. Flooding is when you have to much liquid coming down and at it's extreme the column will be filled with liquid. It is the same problem with weaping but in a way the opposite. There is not enough liquid in the column. A column is easier to operate, answers faster and do not overbalance as easily the closer you are to equilibra.