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Sometimes you do in fact. In IR spectroscopy, the transmittance is commonly plotted instead of absorbance.
However, in most types of spectroscopy, the absorbance is plotted instead. This is due to Beer's Law, which states that the absorbance is directly proportional to the concentration of the sample, the absorptivity of the sample, and the path length (in contrast, the transmittance is exponentially related to those things). Because of the much simpler direct proportionality, absorbance is preferred in many cases.
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What is external calibration and is it the same as the manual calibration of equipment?
Well, external calibration is a method used in analytical chemistry to determine the concentration of an unknown analyte. In essence, you take known concentrations of the analyte and plot it on an absorbance or transmittance graph to get a linear plot. And then you take that linear equation and plug in the absorbance or transmittance value received from the unknown solution and get the concentration. An example of this is if you want to find out the amount of calcium in a vitamin tablet. Dissolve the vitamin tablet and test the solution to get an absorbance value. Then test by the same method various concentrations of a calcium solution, plot this on a graph of absorbance vs. concentration and there yah go.
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Absorbance refers to a measure of the capacity associated with a substance as regards absorption of light of a specified wavelength. Whenever you plot a graph of absorbance vs. concentration a direct relationship should be produced
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