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HCl is a secondary standard solution.It is used in acid-base solution.On the other hand,EDTA is dissolved in pH 8.0.That's a perfect match for the HCl.EDTA chelates divalent cations and helps to inhibit DNAse.Thus,it is possible to get intake DNA.

arif ashraf-biochem-DU-BD

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13y ago

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What is the function of tris and EDTA buffer?

Tris buffers provide a stable pH environment for biochemical reactions, while EDTA chelates metal ions to prevent enzymatic degradation. When used together, the Tris-EDTA (TE) buffer is commonly used for nucleic acid storage and as a buffer in molecular biology applications.


Tris EDTA buffer in plant DNA isolation?

Chelating agent


Role of te in dna extraction?

TE stands for Tris and EDTA. The Tris buffers the water to prevent acid hydrolysis of the DNA/RNA. The EDTA chelates divalent cations that can assist in the degradation of RNA.


How do you make 1X TE buffer?

10 mM Tris pH 7.5 and 1mM EDTA pH 8.0 For 1 L : 10 mL of 1M Tris-Cl pH 7.5 and 2 mL of 500mM EDTA pH 8.0


1X TAE buffer composition?

0.04 M Tris-acetate, 0.001 M EDTA


What are DNA gels?

DNA gels is a term that usually refers to agarose gels, made with TAE (Tris, Acetate, EDTA) or TBE (Tris, Borate, EDTA) buffer. They are the simplest to make and don't contain toxic compounds (unless EtBr is added to the gel).


What is the difference between TAE buffer and TE buffer?

The main difference is in composition. In TE common Tris buffer is bring down to pH 8 with HCl and EDTA is involved but in TAE instead of Tris HCl in TE Tris-acetate buffer is used.


What is STE address or STE buffer?

0.1 M NaCl10 mM Tris-HCl (pH 8.0)1 mM EDTA (pH 8.0)


What is TNE buffer?

tris, EDTA (TE solution) and NaCl, TNE buffer is a buffer solution used in molecular biology, especially for DNA and RNA


Role of tris in TE in DNA elution?

Tris, commonly used as a buffering agent in Tris-EDTA (TE) buffer, helps to maintain the pH stability of the solution during DNA elution. Tris also provides a suitable ionic strength for DNA stability and helps to prevent degradation. It facilitates the solubilization of DNA during elution by providing a mild and stable environment.


Hypocalcemia enhances the excitability of nerves and muscles because of the permeability of the cell membrane to?

cacium


Role of saline tris edta in DNA extraction?

Saline tris EDTA (STE) buffer is used in DNA extraction to provide a suitable environment for DNA stability and prevent DNA degradation. It helps to maintain the pH of the solution, keeps the DNA soluble, and protects it from nucleases that could break it down. Overall, STE buffer helps in the efficient extraction and preservation of DNA from cells.