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Once you have done chromotography and split up all of the amino acids in the protein you will need to measure from the spot where the sample was first placed to the top of the chromatography paper where the solvent stopped being drawn up through it. Next measure from the sample spot to the centre of each of the amino acid spots. Then, to calculate the Rf value divide the amino acid spot distance by the distance the solvent travelled. If you get an answer greater than one then you are dividing the numbers the wrong way round.
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∙ 14y ago
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What is the rf value of cocaine?
Rf is about .45
What is the Rf value of salicylamide?
It will depend on the solvent system you are using.
What is the RF value for aspirin using TLC?
Rf value changes with every experiences..... it depends on what was your solvant, the room température, the chromatograph you used..... that's why you wont find Rf tables in organic chemistry books
What is rf value and how is determine?
the mobility of any component in a particular solvent is referred to as its RF value. Mathematically, RF value = distance travelled by solute/ distance travelled by the solvent
What is the Rf value of anthocyanin?
anthocyanins rf vlaue is o.95
What is the rf value of cocaine?
Rf is about .45
What is the Rf value of salicylamide?
It will depend on the solvent system you are using.
What is rf value of eugenol?
The Rf value depends on the type of plate used and solvents.
How does saturation effect RF value?
Saturation affects the RF value in a significant manner. With saturation of the air, this will result into high RF value which cause poor resolution.Ê
What is the RF value for aspirin using TLC?
Rf value changes with every experiences..... it depends on what was your solvant, the room température, the chromatograph you used..... that's why you wont find Rf tables in organic chemistry books
What is the maximum and RF value can be?
1, if it completely solube in the solvent, it will travel with it, right to the solvent front.
What is rf value and how is determine?
the mobility of any component in a particular solvent is referred to as its RF value. Mathematically, RF value = distance travelled by solute/ distance travelled by the solvent
Would you expect the Rf value of a pigment to be the same if a different solvent were used Explain?
The Rf value would not be the same for every solvent as there are factors that allow each solvent to be unique. The attractive force, particle size and solubility of each solvent will create different results each time.
What is the Rf value of anthocyanin?
anthocyanins rf vlaue is o.95
What is an rf value used for?
The rf value is shorthand for the retention value of a substance. It is used in chromatography to determine the components of an unknown sample.
What is the RF value for red ink blue ink and green ink?
the Rf value value is 8.44 as the particles are lighter than the solvent
What is the relationship between chain length and Rf value?
As a general rule the longer the carbon chain the greater the Rf value.
