If your microscope is a Transmission Microscope then light has to pass through the specimen (that is how the microscope works).
Other kinds of microscopes may not require this.
Light passes through many mediums, but some of the most important are: Water Glass Air & Vacuum And light passes through vacuums the fastest.
Light is scattered as it passes through a prism.
light passes in straight line
Opaque - light does not pass through. Transparent - all light passes through. Translucent - some light passes through.
Light usually passes through a transparent object. Similarly, light passes through the translucent objects but people cannot see through it.
the speed of light, commonly denoted as "c", is constant no matter which medium it passes through.
A material through which nearly all light passes is called a transparent material.
Polarised light!
It happens by the refraction of light.
When light changes direction as it passes through a boundary.
Water is tranparent; light passes through it.
The light will bend as it passes through.