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Why is it necessary to cover the developing chamber tightly during the development of the chromatography?
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Why is it important to cover the developing chamber in paper chromatography?
For thin layer or paper chromatography, this can be a very important step, especially if one or more of the components in the mobile phase have very high vapor pressures (or low boiling points). Without the equilibration, as the mobile phase starts to move up the plate, the more volatile components will evaporate into the air space above the mobile phase faster than the less volatile ones. This is change the composition of the mobile phase (not much, but sometimes enough to change the Rf's of the unknown spots and standards). If the mobile phase consists of only water or perhaps high boiling solvents, the equilibration step is not as important. If the mobile phase contains Methylene chloride, Diethyl ether or similar solvents, then the equilibration step can be very important in terms of reproducibility.
Is Iodine a soild or gas?
Elemental iodine is a solid. But it is a volatile solid in that chemists will use solid iodine in a chamber to "iodine stain" substances in thin layer chromatography. Thus iodine is like having a volatile solid.
What is chamber in science?
what is chamber
What is a sentence using the word chamber?
Chamber mean a room Ex. I hate being in a chamber !
What does a chamber mean?
A chamber can be a room in a house, such as a bed chamber. It can also mean the part of a gun where a bullet lies, such as--> "He chambered a round into the gun."
Why do you need to saturate the chromatography chamber?
1) for uniform development 2) proper elution of sample
What is the purpose of the filter paper inside the developing chamber in chromatography?
Paper is the (solid) stationary phase, 'sucking' up the liquid, mobile (= transporting) phase
Why must the chromatography chamber be closed tightly?
so that the atmosphere in the jar becomes saturated with solvent.
What is the chamber that houses the developing fetus?
The womb (uterus).
How would Rf values changes of Cations if the developing chamber left uncovered in paper Chromatography?
The solvent would keep on evaporating from the chromatogram and as a result it would keep on bringing the cations up, causing an increase in the Rf value.
What problem will ensue if the level of the developing liquid is higher than the applied spot in a TLC analysis?
The effect on chromatographic work if the solvent level in the developing chamber is higher than the spotted sample is a thin layer chromatography. The solvent becomes polar enough and spot will move some distance.
Why is it important to cover the developing chamber in paper chromatography?
For thin layer or paper chromatography, this can be a very important step, especially if one or more of the components in the mobile phase have very high vapor pressures (or low boiling points). Without the equilibration, as the mobile phase starts to move up the plate, the more volatile components will evaporate into the air space above the mobile phase faster than the less volatile ones. This is change the composition of the mobile phase (not much, but sometimes enough to change the Rf's of the unknown spots and standards). If the mobile phase consists of only water or perhaps high boiling solvents, the equilibration step is not as important. If the mobile phase contains Methylene chloride, Diethyl ether or similar solvents, then the equilibration step can be very important in terms of reproducibility.
What would you use in a paper chromatography?
We use several things such as stationary phase which is a paper, mobile phase according to your solute and the mixture to be analysed. All these are placed in a closed chamber.
What is the full form of Ph.D chamber of commerce?
Progress Harmony Development-PHD hope it answers your query
Why all burners must be turned off before any developing chambers?
Because some of the substances used in the chamber are flammable and toxic.
Is Iodine a soild or gas?
Elemental iodine is a solid. But it is a volatile solid in that chemists will use solid iodine in a chamber to "iodine stain" substances in thin layer chromatography. Thus iodine is like having a volatile solid.
How does the Edb plan on developing and expending commerce and industry in the country?
Bahrain is a booming commerce and industry sector. However, the process of development is endless and hence, we have HisExcellancy, Khalid A.Rahman Al Moayyed,Chairman, Bahrain Chamber of Commerce and Industry, as an esteemed member of EDB board of directors, to ensure more growth in the sector.
