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smear should be rinsed with distill water so that all the small particles attach to the smear will washed away such as some time the crystals of dye are attached to the smear which give the illusion of microbial cell some time, distill water is used because it is free from other microbial cell and ions which can harm the smear.

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15y ago
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12y ago

to prevent crystallisation of the stain to

appear on the slide.

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12y ago

the smear is rinsed with water so that the bacteria can take the stain, the bacteria becomes visible under microscope.

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Q: Why is it necessary to excessively rinse the smear with water in experiment identifying bacteria using gram stain?
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