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Let's imagine what happens if you use your contaminated tip from the last dilution:

You wouldn't only transfer cells from the diluted solution, but also take some cells that stick on the tip to your next dilution. So in effect, you will have more cells in the new dilution than you would expect - and your experiments results are improper.

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Wiki User

11y ago
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Wiki User

13y ago

to avoid contamination to the microorganism sample to get the accurateresults of experiments.

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Wiki User

8y ago

Different pipettes have different errors.

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Wiki User

6y ago

The purpose is to avoid any small differences between pipettes volume.

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Tracy Owusu

Lvl 2
1y ago

To avoid contamination to the microorganisms sample to get the accurate results in an experiment.

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Q: Why is it necessary to use a fresh tip for each transfer when making dilutions?
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