It is. Have you taken Microbiology? It is the most widely used isolation technique. The disadvantages to this technique are 1. colonies of several species may present a similar appearance 2. Certain bacteria species won't grow in this environment 3. Difficulty in removing colonies. EMB is the technique that's not commonly used.
what is serial dilution and spread plate technique
Williston et.al and waksman et.al discovered the crowdwed plate technique.
There are mainly two techniques in microbiology in bacterial cell inoculation. The first is when the colony is added to the plate and spread with a spreader across the entire plate in aseptic conditions. The second is called 16-streak and is used to isolate a single colony
Bacterial mixture is transferred to the edge of an agar plate with an inoculating loop and then streaked out in one of several patterns. At some point, individual cells will be removed from the loop and will give rise to separate colonies.source: http://quizlet.com/17578430/micro-lab-unit-1ex-15-16-streak-plate-technique-flash-cards/
Both Spread-plate and pour plate method don't give the same results. Because in the case of spread plate method the inoculmn used for inoculation can't be spread in a exact volume. A little inoculmn remains stick with the spreader after spreading. On the other hand, in pour plate method it doesn't happen. So mostly, through comparing the counts by both methods, less counts are obtained in spread plate method. I am Working as a Sr. Microbiologist in a Biotech company
By using streak plate technique to spread a clinical sample out on the surface of a growth medium individual types of bacteria can be isolated
what is serial dilution and spread plate technique
In the pour plate, the microorganisms will grow within the gel that has been set, and in the spread-plate technique, growth will be on top of the agar gel where it has been spread.
Williston et.al and waksman et.al discovered the crowdwed plate technique.
"Offset printing is a commonly used printing technique where the inked image is transferred (or "offset") from a plate to a rubber blanket, then to the printing surface."
i'ts where you strip in front of a plate
The only way i can think of is using a spread plate technique to observe what grows on an agar plate. Capillary electrophoresis I believe is use to split biological samples (and chemical) based on charge and size.
There are mainly two techniques in microbiology in bacterial cell inoculation. The first is when the colony is added to the plate and spread with a spreader across the entire plate in aseptic conditions. The second is called 16-streak and is used to isolate a single colony
The purpose of the spread-plate technique is to grow and isolate colonies of bacteria. A sample of bacteria is transferred to the agar plate, an environment that provides nourishment for the bacteria to grow. The bacteria sample is applied to the agar plate which a special streaking technique that dilutes the amount of bacteria in each section of the agar plate continuously. This is because if you just swabbed the bacteria onto the plate with no special technique the colonies would grow very densely together and be difficult to study. The streaking technique gradually dilutes the amount of bacteria in each 'quadrant' of the plate, so the last quadrant should have small, isolated colonies that can be easily studied. The spread plate technique is also used for the eneumeration of aerobic microorganisms from the given sample. This can be done by serial diluting the samples, placing 0.1ml of the diluted sample in the middle of an agar plate and spreading the sample over the surface with a help of an L-rod. After the incubation rhe colonies can be counted.
Previous answer: "because of infection" This person is obviously trying to be funny, the right word should be "Contamination", as for spread plate, the bacteria is more exposed to air as it is spread over the agar plate. Therefore, the result might not be accurate, as it might be contaminated. As for the pour plate method, the bacteria is in the agar itself, it is not exposed to air, thus, less risk of getting contaminated.
Bacterial mixture is transferred to the edge of an agar plate with an inoculating loop and then streaked out in one of several patterns. At some point, individual cells will be removed from the loop and will give rise to separate colonies.source: http://quizlet.com/17578430/micro-lab-unit-1ex-15-16-streak-plate-technique-flash-cards/
Robert Koch