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To find out if we have mitochondria, we add DCPIP. The tube that has the most mitochondria will change color most from Blue to Clear. This is because mitochondria has an enzyme called Succinic Dehydrogenase. This enzyme can be picked up by removing (2) Hydrogen atoms and the DCPIP picks those Hydrogen atoms up, becoming DCPIP2.

Note that DCPIP is DARK BLUE, and DCPIP2 is CLEAR. So, when the hydrogen is picked up and DCPIP becomes DCPIP2, the color goes clearer, and so there is mitochondria present.

Info generated from Lab done in class (Mr. Wilson, Bio 181)

Mohamad Ali Zeitoun

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Q: Why is the color change proof of the presence of mitochondria?
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