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In tissue culture, the medium also called the sterile jelly is very essential because it provides all the necessary nutrients , hormones , enzyme which is needed for the growth and development of the tissue and helps it to produce many cells which form a shapeless mass called a 'callus' .This then could be transferred to another jelly which helps it to develop roots and another to develop shoot which then could be transplanted to pots or soil which helps it to mature and grow to form new plant . hence medium is considered very important in tissue culture technique.

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