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because the specimen is always thin
The specimen prepared for a monocular microscope must be very thin so light can pass through it easily. The light then goes through a series of lenses that magnifies the specimen to appear bigger
Thin enough for light to pass through it.
Light microscope works because light goes *through* your specimen. So if the specimen is too thick, then light won't shine through, and you won't see anything.
The speciman you are viewing must be in a vacuum, and be coated with a metal film, therefore the specimen can't be alive and if its a Transmission Microscope- Speciman must be extrememly thin so that the electrons can pass through them. The image is black and white.
because the specimen is always thin
The specimen prepared for a monocular microscope must be very thin so light can pass through it easily. The light then goes through a series of lenses that magnifies the specimen to appear bigger
Thin enough for light to pass through it.
Because light must pass through it.
To see them better
Light microscope works because light goes *through* your specimen. So if the specimen is too thick, then light won't shine through, and you won't see anything.
A specimen being viewed under a microscope should be thin so that light can pass through the specimen. The thinner it is the brighter it will be. A thick specimen will block the light and all you'll get is a dark grey image. Also, more detail can be seen in a thin specimen because there will not be parts in front of each other, blocking the view.
A transmission electron microscope (TEM) is a microscope in which a beam of electrons is transmitted through an ultra-thin specimen, interacting with the specimen as it passes through it.
because the thinner it is the clearer it is too see inside
Cover slip
The speciman you are viewing must be in a vacuum, and be coated with a metal film, therefore the specimen can't be alive and if its a Transmission Microscope- Speciman must be extrememly thin so that the electrons can pass through them. The image is black and white.
For a light transmission microscope, a thin sample is needed in order for the light to pass through the sample from the light source on the other side. However, you can use a dissection microscope (another type of light microscope) which illuminates the sample from above, eliminating the need for a thin sample size. As the name suggests, this is normally used in dissections, and is of low magnification.