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Specimens need to be thin to allow light to pass through them and be able to observe details and structures at a cellular or subcellular level. Thicker specimens would block the light and hinder the ability to visualize the specimen clearly under a microscope. A thin specimen also helps to reduce scattering and distortion of the image.

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1y ago

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How must samples be prepare for observation by electron microscope?

They must be preserved and dehydrated. Once this is accomplished, put the specimen onto a slide, and then the slide is ready to be viewed under the microscope.


How must samples be prepared by observation by an electron microscope?

They must be preserved and dehydrated. Once this is accomplished, put the specimen onto a slide, and then the slide is ready to be viewed under the microscope.


Why must a specimen be thin in order to be viewed under the microscope?

Specimens must be thin in order to be viewed under the microscope because light can only pass through a certain thickness of material. A thin specimen allows light to pass through and interact with the cells, allowing the microscope to produce a magnified image. Thicker specimens would prevent light from passing through and produce a blurry or unreadable image.


Why must a specimen be thin to be viewed under the microscope and why are they sometimes stained with dyes?

Specimens need to be thin to allow light to pass through and enable clear visualization under the microscope. Staining with dyes helps to highlight specific structures or components within the specimen, making them easier to observe and distinguish.


Why must a specimen be very thin to be viewed under a light microscope?

A thin specimen allows light to pass through without distortion, enabling clearer imaging and more precise observations under a light microscope. Thick specimens can scatter light, reducing image quality and making it harder to distinguish details.


Why is the specimen prepared for a monocular microscope must be very thin?

The specimen must be very thin for a monocular microscope because light has difficulty passing through thick specimens, leading to poor image quality and decreased clarity. A thin specimen allows more light to pass through, resulting in a sharper and more focused image when viewed through the monocular microscope.


Why must specimens viewed with a compound microscop be thin?

Specimens viewed with a compound microscope must be thin to allow light to pass through them. This ensures that the light rays can illuminate and pass through the specimen, which is necessary for magnifying the image and producing a clear view under the microscope. Thicker specimens would scatter or block the light, resulting in a blurry or dark image.


How must samples be prepared for observation by an electron microscope?

Samples for observation by an electron microscope are typically prepared by fixing the specimen in a chemical solution, dehydrating it, and then embedding it in a resin for stability. The sample is then sliced into ultrathin sections using a microtome before being stained to enhance contrast. Finally, the prepared sample is placed inside the electron microscope for imaging.


Why should a specimen to be viewed under the microscope must be thin?

Body tissues and bones are opaque beyond a certain thickness. To view fine structures, exceedingly thin samples will be translucent, revealing cell and tissue structure.


Why must specimens viewed with a compound microscope be thin why are they sometimes stained with dyes?

Specimens viewed with a compound microscope need to be thin to allow light to pass through and be focused by the lenses. Staining with dyes increases contrast and visibility of certain structures within the specimen by highlighting specific parts of the sample. This helps to distinguish different components and makes them easier to observe under the microscope.


What must be done there is too much light that illuminates the specimen you are observing under the microscope?

Turn down the light.


What must be done if there is too much light that illuminates the specimen you are observing under the microscope?

tang ina ninyo!!