Specimens viewed with a compound microscope need to be thin to allow light to pass through and be focused by the lenses. Staining with dyes increases contrast and visibility of certain structures within the specimen by highlighting specific parts of the sample. This helps to distinguish different components and makes them easier to observe under the microscope.
molecular biology
Live specimens cannot be used in a transmission electron microscope (TEM) as the high vacuum environment and electron beam would quickly kill any living cells. Instead, biological specimens must be fixed, dehydrated, and stained prior to imaging in a TEM.
Specimens need to be thin to allow light to pass through and enable clear visualization under the microscope. Staining with dyes helps to highlight specific structures or components within the specimen, making them easier to observe and distinguish.
Specimens for examination under an electron microscope are typically fixed with chemicals to preserve cellular structures, dehydrated using ethanol or acetone, and embedded in a resin to provide stability for cutting thin sections. These sections are then stained with heavy metals to enhance contrast and mounted onto a grid for imaging in the electron microscope.
The nucleus of the cheek cell would most likely be visible when viewed through the high-power objective of a compound microscope. It is usually one of the larger and more prominent structures within the cell and can be stained to enhance visibility.
molecular biology
we use sterile water because to avoid false result by microorganism having in the water and same reason for distilled also to avoid impurities like crystals of some metal ions and also to avoid other microorganisms
Live specimens cannot be used in a transmission electron microscope (TEM) as the high vacuum environment and electron beam would quickly kill any living cells. Instead, biological specimens must be fixed, dehydrated, and stained prior to imaging in a TEM.
Specimens are stained with dyes to enhance contrast and visibility under a microscope, allowing specific structures or components to be more easily distinguished. Staining can highlight particular cell types, organelles, or tissues, making it easier to identify and study their morphology and function. Additionally, different dyes can bind to specific cellular components, providing valuable information about the biochemical properties of the specimen.
There there are many. Simple microscope, compound microscope, light microscope, scanning electron microscope, TEMicroscope, Dissection microscope, etc and most of them are used to see small cells that cannot be seen by the naked eye.
Specimens need to be thin to allow light to pass through and enable clear visualization under the microscope. Staining with dyes helps to highlight specific structures or components within the specimen, making them easier to observe and distinguish.
Brightfield microscopy is a common technique where light passes directly through a specimen, illuminating it and creating a bright background. It is often used for observing stained samples with high contrast. Brightfield microscopy is widely employed in biology to visualize cellular structures and study tissue samples.
Specimens for examination under an electron microscope are typically fixed with chemicals to preserve cellular structures, dehydrated using ethanol or acetone, and embedded in a resin to provide stability for cutting thin sections. These sections are then stained with heavy metals to enhance contrast and mounted onto a grid for imaging in the electron microscope.
Under a compound light microscope, you would not be able to see specific organelles like the lysosomes, peroxisomes, endoplasmic reticulum, and Golgi apparatus in an onion cell stained with iodine. These organelles are typically smaller and/or transparent, making them difficult to visualize with this type of microscope.
A ruby is stained by a compound.
Biopsy specimens are often sliced into thin slices, stained, mounted on a glass slide, and examined using a light microscope. Newer sample preparation techniques involve the rapid freezing of the sample and slicing of the still-frozen material.
The clinical specimens that are gram stained are microorganism, bodily fluids and sputum specimen. Gram staining makes it easier for the identification of organisms.