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Can live specimens be used in a TEM microscope?
Live specimens cannot be used in a transmission electron microscope (TEM) as the high vacuum environment and electron beam would quickly kill any living cells. Instead, biological specimens must be fixed, dehydrated, and stained prior to imaging in a TEM.
How specimens are prepared for examination under an electron microscope?
Specimens for examination under an electron microscope are typically fixed with chemicals to preserve cellular structures, dehydrated using ethanol or acetone, and embedded in a resin to provide stability for cutting thin sections. These sections are then stained with heavy metals to enhance contrast and mounted onto a grid for imaging in the electron microscope.
Why must specimens viewed with a compound microscope be thin why are they sometimes stained with dyes?
Specimens viewed with a compound microscope need to be thin to allow light to pass through and be focused by the lenses. Staining with dyes increases contrast and visibility of certain structures within the specimen by highlighting specific parts of the sample. This helps to distinguish different components and makes them easier to observe under the microscope.
What are the characteristic of bright field microscope?
A bright field microscope is a type of light microscope that uses light from below the specimen to illuminate and create contrast with the specimen. It produces a dark image of the specimen against a bright background. Bright field microscopes are commonly used in biology labs for observing stained or naturally pigmented specimens.
What are the key characteristics of an onion cell as observed under a microscope?
The key characteristics of an onion cell observed under a microscope include a rectangular shape, a cell wall that appears as a thin, clear outline, a large central vacuole that takes up most of the cell's space, and small, dark-stained organelles called nuclei.
Can live specimens be used in a TEM microscope?
Live specimens cannot be used in a transmission electron microscope (TEM) as the high vacuum environment and electron beam would quickly kill any living cells. Instead, biological specimens must be fixed, dehydrated, and stained prior to imaging in a TEM.
How specimens are prepared for examination under an electron microscope?
Specimens for examination under an electron microscope are typically fixed with chemicals to preserve cellular structures, dehydrated using ethanol or acetone, and embedded in a resin to provide stability for cutting thin sections. These sections are then stained with heavy metals to enhance contrast and mounted onto a grid for imaging in the electron microscope.
Why must specimens viewed with a compound microscope be thin why are they sometimes stained with dyes?
Specimens viewed with a compound microscope need to be thin to allow light to pass through and be focused by the lenses. Staining with dyes increases contrast and visibility of certain structures within the specimen by highlighting specific parts of the sample. This helps to distinguish different components and makes them easier to observe under the microscope.
What clinical specimens are gram stained?
The clinical specimens that are gram stained are microorganism, bodily fluids and sputum specimen. Gram staining makes it easier for the identification of organisms.
Why are specimens sometimes stained?
we use sterile water because to avoid false result by microorganism having in the water and same reason for distilled also to avoid impurities like crystals of some metal ions and also to avoid other microorganisms
A well stained leaf peel mount when observed under the high power of a microscope shows nuclei in?
A well-stained leaf peel mount observed under high power of a microscope will show nuclei in the plant cells. Nuclei in plant cells appear as darkly stained, round or oval structures within the cytoplasm of the cell, providing important genetic and regulatory functions for the plant cell.
What structure in the cheek cells was stained the darkest?
When stained with iodine and observed under a compound microscope the nucleus will stain the darkest. The cheek cell is frequently employed here as they are simple to collect and tend to take the stain easily.
How are biopsy specimens handled?
Biopsy specimens are often sliced into thin slices, stained, mounted on a glass slide, and examined using a light microscope. Newer sample preparation techniques involve the rapid freezing of the sample and slicing of the still-frozen material.
Why microbes are stained?
microbes are stained so they can be visualised under a light microscope.
What colour does the starch grain be?
Starch grains are typically colorless or white when observed under a microscope. The color may appear dark if stained with certain dyes for visualization purposes.
What are the characteristic of bright field microscope?
A bright field microscope is a type of light microscope that uses light from below the specimen to illuminate and create contrast with the specimen. It produces a dark image of the specimen against a bright background. Bright field microscopes are commonly used in biology labs for observing stained or naturally pigmented specimens.
What are the key characteristics of an onion cell as observed under a microscope?
The key characteristics of an onion cell observed under a microscope include a rectangular shape, a cell wall that appears as a thin, clear outline, a large central vacuole that takes up most of the cell's space, and small, dark-stained organelles called nuclei.
