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agar slant
In pour plate technique the culture to be grwon is pour in melted agar medium, now when we add the diluted sample in agar plate and if the melted agar is very hot, it can lead to the damage of bacterial or fungal cell and may cause in abruption of growth, so the agar is cooled to get the optimum temp. for growth of microbial cell.
to ensure that the cells remain dispersed in an iced water bath.
Agar comes from Red Algae (primarily the Gracilaria genus).It mainly is produed from the red algae Gracilaria lichenoides.This algae is harvested along the western coast of the United States and in parts of Asia. To extract the agar the Algae is boiled, cooled, purified and then dried. The agar is then make into blocks, flakes, or granules.("Agar agar" is usually abbreviated as "agar".)
The agar slant will remain the original color (yellow). However, most labs use the broth.Two media types are commonly used to detect urease activity. Christensen’s urea agar is used to detect urease activity in a variety of microorganisms. Stuart’s urea broth is used primarily for the differentiation of Proteus species.
If you are talking about heating the agar before using it, then you are not talking about making the agar from scratch. You are talking about agar that has already been prepared and has been allowed to solidify in a flask. Heating is necessary because the agar must go from its solid state to a liquid state so that it can bePOURED into a tube where it can solidify inside of the slanted position of the tube.
agar slant
In pour plate technique the culture to be grwon is pour in melted agar medium, now when we add the diluted sample in agar plate and if the melted agar is very hot, it can lead to the damage of bacterial or fungal cell and may cause in abruption of growth, so the agar is cooled to get the optimum temp. for growth of microbial cell.
The ending agar is significantly diluted. If the undiluted agar is pured in sink, it will solidify and block up the sink.
Because the peptone iron agar is used to detect ANAEROBIC bacteria. If you stab it deep into the agar you allow the bacteria to grow in the absence of oxygen. If you only inoculated the surface the bacteria wouldn't grow.
to ensure that the cells remain dispersed in an iced water bath.
Agar comes from Red Algae (primarily the Gracilaria genus).It mainly is produed from the red algae Gracilaria lichenoides.This algae is harvested along the western coast of the United States and in parts of Asia. To extract the agar the Algae is boiled, cooled, purified and then dried. The agar is then make into blocks, flakes, or granules.("Agar agar" is usually abbreviated as "agar".)
Solid medium
Mannitol salt agar inoculated with Micrococcus luteusshowing no fermentation of mannitol (pink medium). The colonies show a yellow pigment which is characteristic of M. luteus.
So that you can be sure that the results are due only to one specific species.
In microbiological testing, a "butt" refers to the bottom of a tube filled with agar. Its most common use is in conjunction with the term "slant". These agar-filled tubes are typically allowed to solidify at an angle so that a larger amount of surface area is available to inoculate by streaking. After incubation, both the slant surface and the butt have to be evaluated because growth on the slanted surface (next to air/oxygen) will indicate one kind of information and the bottom (butt) will indicate additional information about the bacterial growth--usually indicated by a difference in color. For example, in the TSI test (Triple Sugar Iron), a combination where the slant is red and the butt is yellow means that fermentation of glucose has occurred. In a TSI test, the butt is not actually inoculated. However, in a motility test, which does not require a slant, the agar is inoculated by stabbing a straight wire covered with an inoculum of sample down to the bottom of the tube. If the bacteria can grow away from the stab into the agar, they are motile. If growth is confined to the stab length, they are non-motile.
a solid medium