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If you are talking about heating the agar before using it, then you are not talking about making the agar from scratch. You are talking about agar that has already been prepared and has been allowed to solidify in a flask. Heating is necessary because the agar must go from its solid state to a liquid state so that it can bePOURED into a tube where it can solidify inside of the slanted position of the tube.

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Does a test tube hold more liquid than a flask?

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