Use of agar slant

Answer 1

An agar slant is when a test tube is filled with liquid agar and allowed to cool and harden at an angle (slant). Agar is mixed with other nutrients to provide a medium for which bacteria can grow on.

Answer 2

Agar slant can be prepare by following method:

Requirements: nutrient agar, test tubes, pippet, cotton plug, alcohols

Procedure: First sterlize the test tubes having cotton plugs.

Now prepare the nutrient agar medium and autoclave it.

Take the test tube and nutrient agar in LAF and pour the agar in tubes with the help of pippet.

Now keep the agar filled tubes in slanting position until the agar filled is set to form a gel or to solidify.

By this you can prepare agar slants.

NOTE always keep LAF clean with alcohol and do all work carefully without contamination.

Answer 3

Agar slant is used to preserve bacteria cultures. Since the tubes have screw caps, the agar doesn't dry out easily. These tubes also take up little storage space when compared to an agar plate.

Answer 4

Agar plates are made with a shallow layer of agar inside a petri plate. Agar slants are made with a certain amount of agar inside a test tube (deep or shallow) that is set so the top surface has a slant.

Agar plates and slants are used in microbiology for different procedures and tests. Plates have a greater surface area and ease of access; thus they are used for procedures that require counting colonies and seeing their physical proerties. Test tube slants do not dry out as easily as plates and allow for tests in "deep agar". The slant allows for more surface area on the top. The tubes allow chemical tests can take place in a contained area.

Both are used to see the chemical effects and abilities of a microbe's metabolism. For bacteria, testing the properties of an unknown sample is a way to identify the species and strain.

Plates are used to:

• grow and culture bacteria, fungi, animal tissues, or plant tissues
• obtain separated pure cultures of bacteria (plate streaking)
• count colonies from serial dilutions
• test for growth and reactions on certain materials (such as manitol-salt, or blood agar)
• test for bacteria viruses (bacteriophages)
• test for resistance to materials (such as antibiotics) or nutritional needs (this is also used to select for bacteria with certain properties or favor the growth of one type/strain over others)

Test tube slants are used to:

• conduct small scale reactions of a microbe to certain substances (such as H2O2)
• see if a microbe is an obligate aerobe, facultative anaerobe, aerotolerant anaerobe, microaerophile, or obligate anaerobe
• test for reactions to certain materials in the agar (such as citrate agar)
• test for motility of bacteria
• test for production of gases (such as E.coli producing CO2 pockets in the agar)

Answer 5

The purpose of an agar slant is providing storage for bacteria. It is a type of tube with more volume and without being hard to put into a tube stand.

Answer 6

Test tubes that were left at an angle while the agar solidifies, It is used for inoculation of bacterial cultures

Answer 7

For making pure bacterial culture. and further use of that bacteria