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In microbiological testing, a "butt" refers to the bottom of a tube filled with agar. Its most common use is in conjunction with the term "slant". These agar-filled tubes are typically allowed to solidify at an angle so that a larger amount of surface area is available to inoculate by streaking. After incubation, both the slant surface and the butt have to be evaluated because growth on the slanted surface (next to air/oxygen) will indicate one kind of information and the bottom (butt) will indicate additional information about the bacterial growth--usually indicated by a difference in color. For example, in the TSI test (Triple Sugar Iron), a combination where the slant is red and the butt is yellow means that fermentation of glucose has occurred.
In a TSI test, the butt is not actually inoculated.
However, in a motility test, which does not require a slant, the agar is inoculated by stabbing a straight wire covered with an inoculum of sample down to the bottom of the tube. If the bacteria can grow away from the stab into the agar, they are motile. If growth is confined to the stab length, they are non-motile.
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What does it mean to inoculate an agar plate?
to inoculate an agar plate you would place bacteria on the agar to grow
What is the consequence of not spreading the inoculum adequately over the agar surface?
The agar surface of a laboratory dish covers the entire area of the dish. An inadequate spread of inoculate will not give the desired outcome.
Why didn't we inoculate MUG agar directly and bypass lactose broth?
The intended use of Nutrient Agar with MUG is for detecting an enumerating E. Coli in water. This lab wasn't looking specifically and only for e.coli. It was looking for other types of coliforms as well. this is my best guess
What is the reaction of staphylococcus in TSI agar?
acid butt/alkaline slant H2s negative
What is isolation media in microbiology?
The growth media such as nutrient agar or Mckonkey agar is used to isolate microbial cells from the mix culture when inoculate on them. Some time speical media is used which selectivly grow some time of bacteria or a differential media which give different morphology of different bacterial species e.g. blood agar and Mannitol salt agar. It can be done by three ways: the spread plate method, the streak plate method, and the pour plate method.
What does it mean to inoculate an agar plate?
to inoculate an agar plate you would place bacteria on the agar to grow
How do you make a sentence with inoculate?
During the microbiology lab, the students were instructed to inoculate the agar plates with the bacterial culture.
What is the consequence of not spreading the inoculum adequately over the agar surface?
The agar surface of a laboratory dish covers the entire area of the dish. An inadequate spread of inoculate will not give the desired outcome.
Why does a straight inoculating needle used to inoculate an agar deep tube?
because you want to introduces as little air as possible
Why didn't we inoculate MUG agar directly and bypass lactose broth?
The intended use of Nutrient Agar with MUG is for detecting an enumerating E. Coli in water. This lab wasn't looking specifically and only for e.coli. It was looking for other types of coliforms as well. this is my best guess
What is the reaction of staphylococcus in TSI agar?
acid butt/alkaline slant H2s negative
How do you spell innoculate?
The correct spelling is inoculate.
What is isolation media in microbiology?
The growth media such as nutrient agar or Mckonkey agar is used to isolate microbial cells from the mix culture when inoculate on them. Some time speical media is used which selectivly grow some time of bacteria or a differential media which give different morphology of different bacterial species e.g. blood agar and Mannitol salt agar. It can be done by three ways: the spread plate method, the streak plate method, and the pour plate method.
What is a sentence using the word inoculate?
You must inoculate your pets against rabies. The killed cowpox vaccine was used to inoculate people against smallpox.
Why the slant portion is blue but the butt remains green at the microorganism grows on Simmon's citrate agar?
Fermenting citrate
What is a good sentence for the word inoculate?
To inoculate means to inject a micro-organism into something, as in vaccines or inoculating plants with certain experimental micro-organisms. The doctor will inoculate you against smallpox with a vaccine.It can also mean to make someone become full of a certain quality. The politician will inoculate everyone with the idea that freedom must be fought for.I will inoculate the next patient with a dose of oral polio vaccine.Definition: (v.) to treat with the objective of producing resistance to a disease.Sentence: 'The doctor gave Joe a shot to inoculate him for the flu.'
What is the best biochemical test to identify gram negative and H2S bacilli?
Gram Stain to determine gram negative and shape or streak on a Agar plate selective for gram negative such as Desoxycholate Agar (DES). A SIM (Sulfur, Indole, Motility) Test or a Kiliger's Iron Agar (KIA) Test can confirm the production of H2S. *I would choose Streaking on DES to isolate the gram Negative bacteria, and then stab inoculate a SIM tube. Black precipitate confirms H2S production positive.
