answersLogoWhite

0

In microbiological testing, a "butt" refers to the bottom of a tube filled with agar. Its most common use is in conjunction with the term "slant". These agar-filled tubes are typically allowed to solidify at an angle so that a larger amount of surface area is available to inoculate by streaking. After incubation, both the slant surface and the butt have to be evaluated because growth on the slanted surface (next to air/oxygen) will indicate one kind of information and the bottom (butt) will indicate additional information about the bacterial growth--usually indicated by a difference in color. For example, in the TSI test (Triple Sugar Iron), a combination where the slant is red and the butt is yellow means that fermentation of glucose has occurred.

In a TSI test, the butt is not actually inoculated.

However, in a motility test, which does not require a slant, the agar is inoculated by stabbing a straight wire covered with an inoculum of sample down to the bottom of the tube. If the bacteria can grow away from the stab into the agar, they are motile. If growth is confined to the stab length, they are non-motile.

User Avatar

Wiki User

14y ago

What else can I help you with?

Continue Learning about Biology

What does it mean to inoculate an agar plate?

Inoculating an agar plate refers to transferring microorganisms onto the surface of the agar using a sterile inoculating loop. This allows the microorganisms to grow and form visible colonies that can be studied or identified.


What is a sentence using the word inoculate?

You must inoculate your pets against rabies. The killed cowpox vaccine was used to inoculate people against smallpox.


What is the consequence of not spreading the inoculum adequately over the agar surface?

Not spreading the inoculum adequately over the agar surface can lead to inaccurate results in microbiological studies. Uneven distribution may result in uneven growth of microbial colonies, making it difficult to accurately interpret and analyze the data. This can impact the reliability and reproducibility of experimental outcomes.


What is the reaction of staphylococcus in TSI agar?

Staphylococcus typically shows no reaction in Triple Sugar Iron (TSI) agar due to its inability to ferment lactose or produce hydrogen sulfide. Staphylococcus colonies on TSI agar appear as small, round, and colorless colonies without any color changes in the medium.


What is isolation media in microbiology?

The growth media such as nutrient agar or Mckonkey agar is used to isolate microbial cells from the mix culture when inoculate on them. Some time speical media is used which selectivly grow some time of bacteria or a differential media which give different morphology of different bacterial species e.g. blood agar and Mannitol salt agar. It can be done by three ways: the spread plate method, the streak plate method, and the pour plate method.

Related Questions

How do you make a sentence with inoculate?

During the microbiology lab, the students were instructed to inoculate the agar plates with the bacterial culture.


What does it mean to inoculate an agar plate?

Inoculating an agar plate refers to transferring microorganisms onto the surface of the agar using a sterile inoculating loop. This allows the microorganisms to grow and form visible colonies that can be studied or identified.


Why straight neddle are use to inoculate agar deep tube?

Straight needles are used to inoculate agar deep tubes because they can easily penetrate the agar without causing damage, ensuring that the inoculum is delivered to the desired depth within the tube. Straight needles also provide precision and control during the inoculation process, helping to create a uniform distribution of the sample within the tube.


Why does a straight inoculating needle used to inoculate an agar deep tube?

because you want to introduces as little air as possible


How do you spell innoculate?

The correct spelling is inoculate.


What is a sentence using the word inoculate?

You must inoculate your pets against rabies. The killed cowpox vaccine was used to inoculate people against smallpox.


What is the consequence of not spreading the inoculum adequately over the agar surface?

Not spreading the inoculum adequately over the agar surface can lead to inaccurate results in microbiological studies. Uneven distribution may result in uneven growth of microbial colonies, making it difficult to accurately interpret and analyze the data. This can impact the reliability and reproducibility of experimental outcomes.


What is the protocol for culture of fungi on rice starch agar media?

* Prepare a Rice Starch Agar Media (RSA) * Sterilize the media , glasswares , pipette etc. by autoclave at 121 degree C temp. and 15lbs pressure for 15 mins. * take the media and other things to the aseptic chamber. * Pour the media in the petri plate. * Allow it to solidify. * Inoculate the sample with the help of inoculating loop on the media. * Incubate it at RT for 3-7 days for fungal growth. * Obeserve for the shape, size, appearance of the colonies.


What is a good sentence for the word inoculate?

To inoculate means to inject a micro-organism into something, as in vaccines or inoculating plants with certain experimental micro-organisms. The doctor will inoculate you against smallpox with a vaccine.It can also mean to make someone become full of a certain quality. The politician will inoculate everyone with the idea that freedom must be fought for.I will inoculate the next patient with a dose of oral polio vaccine.Definition: (v.) to treat with the objective of producing resistance to a disease.Sentence: 'The doctor gave Joe a shot to inoculate him for the flu.'


Agar agar grain found in Germany?

Agar, or agar-agar, is not a grain, but rather an extract of seaweed. Agar translates to German as Agar-Agar Try whole- or health-food stores


Example of the word inoculate in a sentence?

it is a inculate


To inoculate for example against smallpox?

varicella