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To grow E. coli in a petri dish, first prepare a nutrient agar medium by mixing agar with a nutrient broth, then autoclave to sterilize. Once cooled to about 50°C, pour the agar into sterile petri dishes and allow it to solidify. Inoculate the agar surface with E. coli using a sterile loop or swab, then incubate the plates upside down at 37°C for 24 hours. After incubation, observe the growth of colonies.

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