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* Prepare a Rice Starch Agar Media (RSA)

* Sterilize the media , glasswares , pipette etc. by autoclave at 121 degree C temp. and 15lbs pressure for 15 mins.

* take the media and other things to the aseptic chamber.

* Pour the media in the petri plate.

* Allow it to solidify.

* Inoculate the sample with the help of inoculating loop on the media.

* Incubate it at RT for 3-7 days for fungal growth.

* Obeserve for the shape, size, appearance of the colonies.

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