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acid butt/alkaline slant H2s negative

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Q: What is the reaction of staphylococcus in TSI agar?
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What agar is the best for staphylococcus aureus?

Mannitol salt agar is one of the best agar fro isolation of Staphylococcus aureus


What is the pH indicator in TSI agar?

Phenol Red


Does Staphylococcus grow on non crystal violet MacConkey agar?

Staphylococcus will not grow on Mac since crystal violet inhibits the growth of Gram positivesfmchinea: Actually some species of staphylococcus grow on MacConkey's plate including Staphylococcus aureus.


What color would tsi media be after 48 hours incubation with escherichia coli?

TSI media for E. coliGlc (+) butter yellowLac/Suc (+) yellowH2S (-) no black precipitateGas (+) agar may be lifted


What reaction would saccharolytic bacterium give in a TSI tube?

The saccharolytic bacterium in a TSI tube would liberate sulfates inside the tube turning it yellow. The TSI tube is triple sugar iron agar, which tells us if the bacterium can live in glucose, sucrose or lactose.


Does klebsiella ferment lactose and or produce gas?

Yes. Incubation in TSI slanted agar tests positive for both characteristics.


What is a Triple sugar iron agar test?

TSI test used to differentiate the enterobacteriaceae family members like e.coli and enterobacter and pseudomonas


How do you different colony between bacillus subtilis and staphylococcus aureus in MYP agar?

Bacillus subtilis colonies on MYP are big, yellow with rough edges while Staphylococcus aureus colonies are also yellow but small with smooth edges


Which medium is used for isolation and identification of staphylococcus aureus?

agar blood media.It make plaques due to lysis of red bllod cells by wich it is identificated.


What are the general characteristics of staphylococcus Aureus?

Gram + coccus. Positive methanol test. Yellow on agar.


What are the colonial features of staphylococus aureus in nutrient agar?

* Colonies are circular, 2-3 nm in diameter with a smooth shiny surface when grown on nutrient agar,milk agar or blood agar for 24 h at 37 degrees * Colonies are often pigmented,though a few strains are unpigmented Got the answers from here http://www.geocities.com/SouthBeach/Port/3008/staphyl.html


What is an agarslant?

Agarslant is a means whereby agar is created. It is also known as a TSI or Triple Sugar Iron Test. It is a process whereby agar is cooled in a slanted position. Here's what happens: agar is mixed in a test tube with 1% lactose, 1% sucrose, 0.1% glucose, sodium thiosulfate, and ferrous sulfate or ferrous ammonium sulfate. This causes the agar to react in such a way so that it cools in a slanted position.