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That depends on your protocol...it really won't make much difference in the final product because autoclaving will liquify the agar if it were already solidified so, you'll need to let them cool and re-solidify before use. If you are transporting a bunch of liquified slants to the autoclave you'll likely have a mess as the media sloshes back and forth if the lids are off or loose. Of course, autoclaving them with the lids on tight is generally a bad idea.

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14y ago

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