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What is azocasein?
Azocasein is a chemically modified protein that was designed as a substrate for quantative assay of proteolytic enzyme activity. Hope that helped!!
What is the meaning of plate assay?
plate assay is the process of observation of colonial physiology and morphology of bacterial cell on agar plate. It helps in differentiation of different species of microbes in many process as in identification of enzyme producing or antibiotic producing organism
What is an assay?
An assaying is a trial by assay, thorough examination and determination.
Describe the mechanism of Enzyme linked Immunosorbent Assay ELISA?
Enzyme Linked Immuno Sorbant Assay (ELISA) is so sensitive because of the detection method, i.e. using antibody, and visual detection. A positive control is needed because of the relative selectivity of the antibody. It can always bind to other stuff and give artifactually high values. Nonspecific, unoccupied binding sites in the microtiter plate (as well as other places) have to be blocked or they will give a signal as though they were the analyte of interest.
What is an assayer?
A method used for measuring enzymatic activity.
What has the author Laura Heggie written?
Laura Heggie has written: 'Approaching an RT-PCR Assay to Analyse Gene expression in Chilling-Stressed Rhododendron: Partial Cloning of an Ascorbate Peroxidase Gene and Enzyme Activity Studies'
What has the author Sakina Zerizer written?
Sakina Zerizer has written: 'Comparative biochemical study of peroxidase assay systems'
When can refrigeration not applicable to preserve sample for enzymatic assay?
Refrigeration is not applicable to preserve sample for enzymatic assay because enzymes may lose their activity at extremely low temperatures as well. This may account for storing enzymes at 5° C or below without affecting the enzymatic activity permanently. (Anubhav, 2012)
What does Elisa stand for?
Enzyme Linked Immunosorbant Assay.
What is the full form of ELISA?
enzyme linked immunosorbant assay
What does the medical abbreviation ELISA mean?
Enzyme linked immunosorbent assay. It's a kind of test process.It is a medical technique for finding the presence of an antibody or an antigen in a sampling.Literally it stands for Enzyme Linked Immunosorbent Assay.
What is the ELISA-ACT short for?
Enzyme-linked immunoserological assay activated cell test
What has the author Margaret Jo Whipple written?
Margaret Jo Whipple has written: 'Development of an enzyme-linked immunosorbent assay for the serologic diagnosis of bovine adenovirus type 3' -- subject(s): Enzyme-linked immunosorbent assay, Adenoviruses, Viruses, Cattle
Why you are using enzyme blank and substrate blank instead of using a single blank to find out the enzyme activity?
I am working on pectinase enzyme assay. I incubated 900 ul of substrate for 10 minutes in the water bath, followed by adding 2ml of DNSA reagent, then 100ul of enzyme extract added finally i read the absorbance @ 540 OD. However the values are high. How can I troubleshoot high enzyme blank values in Pectinase assay ?
What is azocasein?
Azocasein is a chemically modified protein that was designed as a substrate for quantative assay of proteolytic enzyme activity. Hope that helped!!
What has the author Gregory J Rutherford written?
Gregory J. Rutherford has written: 'Development of an enzyme-linked immunosorbent assay to detect antibodies against Bacillus thuringiensis subspecies israelensis in Mallard ducks (Anas platyrhynchos)' -- subject(s): Enzyme-linked immunosorbent assay, Bacillus thuringiensis, Mallard, Effect of pesticides on
What is the meaning of plate assay?
plate assay is the process of observation of colonial physiology and morphology of bacterial cell on agar plate. It helps in differentiation of different species of microbes in many process as in identification of enzyme producing or antibiotic producing organism
