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Q: Why pyrene using for wavelength accuracy for hplc calibration?
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How can accuracy be ensured when using a pH meter?

To ensure accuracy when using a pH meter, it is important to calibrate the meter before each use using buffer solutions of known pH values. The meter should also be properly maintained and stored according to the manufacturer's guidelines. Additionally, performing multiple measurements and averaging the results can help increase accuracy.


What are the precautions to be taken while using UV spectrophotometer?

One should not touch the optical assembly with bear hands. Optical surface of cuvettes should not be touched with fingers to avoid erratic reading. Always ensure effectiveness of silica gel kept in instrument. Preserve the instrument from dust particles. Never wash the cuvettes with chromic acid. Wash the cuvette with the distilled water and then with methanol before storage. Check the calibration status before using the instrument and ensure the timely calibration. Do not overfill the cuvettes. Perform the visual inspection of cuvettes for suitability.


Using planck's law determine the wavelength of maximum emission intensity for blackbody with a temperature of 6000K?

Use the formula Lamdamax*T = b Where b is Wein's constant, 2.898 x 10^-3 m K you get Lamdamax = 4.97 X 10^-7 m this wavelength represents UV light.


What are the advantages and disadvantages of using a pH strips?

Advantages to using pH strips is that the user does not have to determine which indicator to use for a sample, they are inexpensive and it's a faster way to receive results than using indicators. Disadvantages to using pH strips is that the accuracy is lower and the solution should be only clear and colorless.


What is the principle of conductivity?

The common laboratory conductivity meters employ a potentiometric method and four electrodes. Often, the electrodes are cylindrical and arranged concentrically. The electrodes are usually made of platinum metal. An alternating current is applied to the outer pair of the electrodes. The potential between the inner pair is measured. Conductivity could in principle be determined using the distance between the electrodes and their surface area using the Ohm's law but generally, for accuracy, a calibration is employed using electrolytes of well-known conductivity. Industrial conductivity probes often employ an inductive method, which has the advantage that the fluid does not wet the electrical parts of the sensor. Here, two inductively-coupled coils are used. One is the driving coil producing a magnetic field and it is supplied with accurately-known voltage. The other forms a secondary coil of a transformer. The liquid passing through a channel in the sensor forms one turn in the secondary winding of the transformer. The induced current is the output of the sensor.

Related questions

What is wavelength accurecy?

wavelength accuracy is described as follows: "Verify the wavelength scale using the absorption maxima of holmium perchlorate solution . The permitted tolerance is ±1 nm for the ultraviolet range and ±3 nm for the visible range".


Why we are using inductive loads for calibration?

You need to specify the kind of calibration you are asking, to get the right answer


What is the greatest concern for scientists using the internet?

accuracy


What is the formula for period using wavelength and speed?

Period = wavelength/speed


Why do you use the wavelength with the maximum absorbance in spectroscopy?

Short answer:Using the maximum wavelength gives us the best results. This is because at the peak absorbance, the absobance strength of light will be at the highest and rate of change in absorbance with wavelength will be the smallest. Measurements made at the peak absorbance will have the smallest error.Long answer: It really depends on what is the largest source of error. Taking the readings at the peak maximum is best at low absorbance, because it gives the best signal-to-noise ratio, which improves the precision of measurement. If the dominant source of noise is photon noise, the precision of absorbance measurement is theoretically best when the absorbance is near 1.0. So if the peak absorbance is below 1.0, then using the peak wavelength is best, but if the peak absorbance is well above 1.0, you might be better off using another wavelength where the absorbance is closer to 1. Another issue is calibration curve non-linearity, which can result in curve-fitting errors. The non-linearity caused by polychromatic light is minimized if you take readings at either a peak maximum or a minimum, because the absorbance change with wavelength is the smallest at those wavelengths. On the other hand, using the maximum increases the calibration curve non-linearity caused by stray light. Very high absorbances cause two problems: the precision of measurement is poor because the transmitted intensity is so low, and the calibration curve linearity is poor due to stray light. The effect of stray light can be reduced by taking the readings at awavelength where the absorbance is lower or by using a non-linear calibration curve fitting technique. Finally, if spectral interferences are a problem, the best measurement wavelength may be the one that minimizes the relative contribution of spectral interferences (which may or may not be the peak maximum). In any case, don't forget: whatever wavelength you use, you have to use the exact same wavelength for all the standards and samples. See http://terpconnect.umd.edu/~toh/models/BeersLaw.htmlTom O'HaverProfessor Emeritus


What are the advantages of using instrumental methods of analysis?

Disadvantages of titrimetryA. Capable of higher degree of precision and accuracy than instrumental methods of analysisB. Analysis can be automatedC. Cheap to perform and do not require specialized apparatusD. They are absolute methods and are not dependent on the calibration of the instrumentE. None


What do you mean by using the word wavelength?

The word "wavelength" refers to the frequency of a wave. Wavelength is measured in hertz; the number of vibrations per second.


What is calibration equipment using high pressure?

Pressure is usually measures and calibrated in "bars".


Why you are using kcl in the calibration of uv visible spectrophotometer?

how do you determine degree of deacetylation of chitosan using UV -vis spectrophotometer


Why you are using caffeine in hplc for calibration?

Caffeine having different wavelents it having 2 maximas and 1 minima. 1st maxima is 205nm 2nd maxima is 273nm minima is 245nm and it is primary reference standard and also suggested in pharmacopiea. -Rajesh,Orchid


How do you calibrate a sphygomanometer?

I used calibration standards available from the manufacturer, which in my case was Brookfield. The maker of your viscometer should offer them. If not, search the internet using "viscometer calibration standard solutions" as your search term.


Which is the best calibration management software?

The process of calibrating different equipment can be processes to standard tolerance values. This process is not been an always easy to calibrate a large amount of equipment. By using this calibration management software of Qualityze, you can ensure equipment precision by coordinating and executing calibration procedures which comply with the standard values. This software enables you to standardize the calibration process in your organization and store all your device calibration records on a centralized platform. Calibration management software has the solution which helps to create the calibration criteria and tasks with the specific instructions to the right person for review, approval, and completion.